Invitrogen

PYGL Polyclonal Antibody

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PYGL Antibody in Western Blot (WB) — Western blot analysis of PYGL was performed by loading whole-cell lysates of four different human cell lines. PYGL was detected at approximately 95 kDa by using a PYGL polyclonal Antibody (Product # PA5-50479) at a concentration of 2.0 µg/mL in Blocking Buffer overnight at 4°C on a rocking platform, followed by a 1-hour room-temperature incubation of a donkey anti-rabbit IgG antibody at a dilution of 1:5,000. Data courtesy of Thermo Scientific KOL Program.

产品信息

PA5-50479

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫组化（石蜡） (IHC (P))	1:30-1:150	-

产品规格
种属反应	Human
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Fusion protein corresponding to residues near the C terminal of human phosphorylase, glycogen, liver 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	2 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4, with 40% glycerol
内含物	0.05% sodium azide
保存条件	-20°C
RRID	AB_2635932

产品详细信息

The antibody detects endogenous levels of total PYGL protein.

靶标信息

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: glycogen liver; Glycogen phosphorylase, liver form; GPLL

基因别名: GSD6; PYGL

UniProt ID: (Human) P06737

Entrez Gene ID: (Human) 5836

分子生物学功能: glycosyltransferase transferase metabolite interconversion enzyme

功能

glycogen metabolic process glycogen catabolic process 5-phosphoribose 1-diphosphate biosynthetic process glucose homeostasis necroptotic process

参与通路

purine nucleobase binding protein binding ATP binding glucose binding drug binding glycogen phosphorylase activity AMP binding vitamin binding pyridoxal phosphate binding bile acid binding protein homodimerization activity

PYGL Polyclonal Antibody

产品信息

PA5-50479

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

篇参考文献 (0)

生物信息学

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