Rockland

Pancreatic Islet Lysate Polyclonal Antibody

Name: Pancreatic Islet Lysate Polyclonal Antibody
Brand: Rockland
SKU: 214-A01-118

查看其他9个PPY抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Immunogen image

Immunogen Image Pancreatic Islet Lysate Polyclonal Antibody

产品信息

214-A01-118

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

产品规格

种属反应

Pig

宿主/亚型

Dog

分类

Polyclonal

类型

Antibody

抗原

Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Ion-exchange chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum. Cross reactivity was determined by immunoblot (western) to be minimal against porcine kidney and porcine liver cell lysates.

靶标信息

The PPY gene encodes pancreatic polypeptide, a peptide hormone involved in the regulation of appetite and energy balance. The origin of the PPY gene is linked to a tandem duplication of the PYY gene, occurring concomitantly with the emergence of tetrapods, leading to the distinct PPY function within the neuropeptide Y family. In primates and ungulates, a gene duplication event resulted in the formation of a PYY2-PPY2 gene cluster, highlighting the evolutionary complexity of these genes. The PPY gene product is secreted by gamma-cells in the pancreas, localized primarily in the islet periphery, and plays a crucial role in metabolic homeostasis by interacting with receptors in the central nervous system to modulate satiety signals postprandially. PPY's function is linked to its inhibition of AGRP/NPY neurons which decreases their suppressive effect on POMC neurons, enhancing POMC activity and contributing to appetite suppression. This intricate regulation underscores the importance of PPY in dietary intake and metabolic disorders, with ongoing research exploring its potential therapeutic applications.

仅用于科研。不用于诊断过程。未经明确授权不得转售。