Nordic

Pepsin Polyclonal Antibody

Name: Pepsin Polyclonal Antibody
Brand: Nordic
SKU: NE117-7S

查看其他13个PGA5抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

产品信息

NE117-7S

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

点杂交 (DB)

Assay-dependent

产品规格

种属反应

Pig

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Porcine Pepsin

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

10 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.

Cross-reactivities against enzymes of other sources may occur but have not been determined.

Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

靶标信息

PGA5: Pepsinogen 5, group I (pepsinogen A). Pepsinogens are the inactive precursors of pepsin, the major acid protease found in the stomach. Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. PGA5 is a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates. PGA5 is inhibited by ovUS-1, a uterine serpin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。