Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Perlecan Monoclonal Antibody (A7L6)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 5
Perlecan Antibody (MA1-06821) in ICC/IF
Immunofluorescence analysis of HSPG2 was performed using 70% confluent log phase HUVEC and SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Perlecan Monoclonal Antibody (A7L6) (Product # MA1-06821, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Prod... View More
产品信息
MA1-06821
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Fish,
Human,
Mouse,
Rat
已发表种属
Bovine,
C. elegans,
Human,
Mouse
宿主/亚型
Rat
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
A7L6
抗原
High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
purified
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-06821 detects large heparan sulphate proteoglycan in human, mouse, bovine, cod, wolffish, and rat samples.
MA1-06821 has sucessfully been used in Western blotting, immunocytochemistry, immunoprecipitation, and immunohistochemistry procedures. By Western blot it detects a >400 kDa band representing large HSPG.
The MA1-06821 immunogen is a high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
靶标信息
Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); HSPG; Perlecan; perlecan proteoglycan; PLC; RP11-132G19.2
基因别名: AI852380; HSPG; HSPG2; Pcn; per; PLC; PRCAN; SJA; SJS; SJS1
UniProt ID: (Human) P98160, (Mouse) Q05793
Entrez Gene ID: (Human) 3339, (Bovine) 444872, (Mouse) 15530, (Rat) 313641
retinoid metabolic process
angiogenesis
glycosaminoglycan biosynthetic process
glycosaminoglycan catabolic process
extracellular matrix disassembly
extracellular matrix organization
glycosaminoglycan metabolic process
lipoprotein metabolic process
cellular protein metabolic process
endochondral ossification
chondrocyte differentiation
brain development
protein localization
embryonic skeletal system morphogenesis
cardiac muscle tissue development
cartilage development involved in endochondral bone morphogenesis
