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Agrisera
Phly/DNA photolyase (At4g25290) Polyclonal Antibody
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Phly/DNA photolyase (At4g25290) Antibody (AS15-2864) in WB
2,5 µg of total protein from Arabidopsis thaliana wild type darkness (1), wild type light (2) and insertion mutants: SALK_056328 C darkness (3), SALK_056328 C light (4), extracted with 0.1 M Tris-HCl p H 8.5,4% SDS, 2% (v/v) 2-mercaptoethanol, 2 m M phenylmethylsulphonyl fluoride and denatured with LaemmLi buffer at 95 o C for 10 min were separated on 12% SDS-PAGE and blotted 2 h to PVDF using semi-dry tran... View More
产品信息
AS15-2864
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana DNA photolyase, located towards C-terminal part of this protein.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
DNA photolyase genes encode flavin-dependent, blue/near-UV light-responsive enzymes that localize predominantly to the nucleus where they act directly on UV-damaged DNA, binding cyclobutane pyrimidine dimers or (6-4) photoproduct lesions and using absorbed photon energy to catalyze light-driven reversal of these lesions (photoreactivation), thereby restoring normal base pairing and genome integrity. Photolyases are typically monomeric proteins built around a conserved photolyase homology region comprising an N-terminal alpha/beta domain and a helical domain that together form a substrate-binding cavity and a cofactor pocket; they bind flavin adenine dinucleotide (FAD) as the catalytic chromophore and, in many species, a second light-harvesting antenna cofactor (commonly a pterin such as 5,10-methenyl-tetrahydrofolate) that broadens light absorption and transfers excitation energy to FAD. In organisms lacking true photolyase activity, related photolyase/cryptochrome family proteins retain the characteristic photolyase-like fold and FAD binding but have structural features that disfavor lesion binding and instead function primarily in blue-light signaling and/or circadian clock regulation rather than DNA repair.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 6-4 PHOTOLYASE; F24A6.130; F24A6_130; UV REPAIR DEFECTIVE 3
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