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Invitrogen
Phospho-4EBP1 (Thr37) Recombinant Rabbit Monoclonal Antibody (52H37L2)
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
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Phospho-4EBP1 (Thr37) Antibody (700238) in ICC/IF
Immunofluorescent analysis of 4E-BP1 (pT37) was done on 70% confluent log phase U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with 4E-BP1 (pT37) Recombinant Rabbit Monoclonal Antibody (Product # 700238) at 2 µg-4 µg in 1% BSA and incubated... View More
产品信息
700238
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
52H37L2
抗原
A peptide corresponding to amino acids 31-41 of Q13541.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532302
产品详细信息
This antibody is predicted to react with bovine, equine, mouse, opossum, rat and zebrafish based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E, which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 4E-BP1; eIF4E-binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC431; MGC4316; P/OKCL.6; PHAS-1; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1
基因别名: 4E-BP1; 4EBP1; BP-1; EIF4EBP1; PHAS-I
UniProt ID: (Human) Q13541
Entrez Gene ID: (Human) 1978
G1/S transition of mitotic cell cycle
IRES-dependent translational initiation
response to ischemia
insulin receptor signaling pathway
lung development
negative regulation of protein complex assembly
TOR signaling
response to ethanol
positive regulation of mitotic cell cycle
negative regulation of translational initiation
cellular response to hypoxia
