应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
1-2 µg/mL | 查看1篇已发表文章 1篇已发表文章 |
免疫组化(石蜡) (IHC (P)) |
1:200-1:250 | - |
免疫细胞化学 (ICC/IF) |
2 µg/mL | - |
染色质免疫沉淀 实验 (ChIP) |
Assay-Dependent | - |
Luminex (LUM) |
- | 查看1篇已发表文章 1篇已发表文章 |
产品规格 | |
---|---|
种属反应 |
Chicken, Human, Mouse, Zebrafish |
已发表种属 |
Human |
宿主/亚型 |
Mouse / IgG1 |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
104A282 |
抗原 |
This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 473 of human Akt1. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
0.5 mg/mL |
纯化类型 |
Protein G |
保存液 |
PBS |
内含物 |
0.05% sodium azide |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_557538 |
MA1-20325 detects phospho-AKT1 (pS473) in human and mouse samples.
AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: AKT1 kinase; AKT1m; Akt1m protein; C-AKT; CAKT; PKB; PKB alpha; PKBG; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; related to A and C kinases; serine-threonine protein kinase; serine/threonine protein kinase; Thymoma viral proto-oncogene; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
基因别名: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA
UniProt ID: (Human) P31749, (Mouse) P31750
Entrez Gene ID: (Chicken) 395928, (Human) 207, (Mouse) 11651, (Zebrafish) 101910198
分子生物学功能:
non-receptor serine/threonine protein kinase
protein modifying enzyme