Invitrogen

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7个已发表图片

10篇引用文献

查看其他15个AMPK alpha-1,2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Product Image Gallery

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB) — Western blot analysis of Phospho-AMPK-alpha 1,2 (Thr183, Thr172) was performed by loading 30 µg of THP-1 cell lysates from cells either treated with a vehicle control (C, left lane) or with 100 nM PMA (right lane) for 15 minutes, onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBST for 1 hour at room temperature. The membrane was probed with a Phospho-AMPK alpha-1,2 (Thr183, Thr172) polyclonal antibody (Product # PA5-17831) at a dilution of 1:1000 for overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG at a dilution of 1:40,000 for 1 hour at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Phospho-AMPK alpha-1,2 (Thr183, Thr172) antibody (Product # PA5-17831) shows increased expression of proteins phosphorylated at the threonine residues in A-431 and Hep G2 treated with Metformin and U-87 MG treated upon with AICAR treatment. {TM}

产品信息

PA5-17831

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:1,000	查看10篇已发表文章 10篇已发表文章

产品规格
种属反应	Bovine, Chicken, Human, Mouse, Non-human primate, Pig, Rat
已发表种属	Human, Mouse, Pig, Plant, Sheep
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic phosphopeptides corresponding to residues surrounding Thr183 of human AMPKa 1 and Thr172 of AMPKa 2
偶联物	Unconjugated
形式	Liquid
纯化类型	Antigen affinity chromatography
保存液	0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/mL BSA
内含物	no preservative
保存条件	-20°C
RRID	AB_10983715

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The combination of lactoferrin and linolenic acid inhibits colorectal tumor growth through activating AMPK/JNK-related apoptosis pathway.

PeerJ

Yao Q,Li H,Fan L,Huang S,Wang J,Zheng N

PA5-17831 was used in Western Blot to investigate if lactoferrin + linolenic acid combination inhibites HT29 tumor formation by activating the AMPK/JNK related pathway.

Thu Jun 17 00:00:00 EDT 2021

The combination of lactoferrin and linolenic acid inhibits colorectal tumor growth through activating AMPK/JNK-related apoptosis pathway.

PeerJ

Yao Q,Li H,Fan L,Huang S,Wang J,Zheng N

PA5-17831 was used in Western Blot to investigate if lactoferrin + linolenic acid combination inhibites HT29 tumor formation by activating the AMPK/JNK related pathway.

Thu Jun 17 00:00:00 EDT 2021

SIRT6 enhances oxidative phosphorylation in breast cancer and promotes mammary tumorigenesis in mice.

Cancer & metabolism

Becherini P,Caffa I,Piacente F,Damonte P,Vellone VG,Passalacqua M,Benzi A,Bonfiglio T,Reverberi D,Khalifa A,Ghanem M,Guijarro A,Tagliafico L,Sucameli M,Persia A,Monacelli F,Cea M,Bruzzone S,Ravera S,Nencioni A

PA5-17831 was used in Western Blotting to determine the effects of the enzymatic activity of SIRT6.

Fri Jan 22 00:00:00 EST 2021

SIRT6 enhances oxidative phosphorylation in breast cancer and promotes mammary tumorigenesis in mice.

Cancer & metabolism

PA5-17831 was used in Western Blotting to determine the effects of the enzymatic activity of SIRT6.

Fri Jan 22 00:00:00 EST 2021

Mitochondria as Target for Tumor Management of Hemangioendothelioma.

Antioxidants & redox signaling

Gordillo GM,Biswas A,Singh K,Sen A,Guda PR,Miller C,Pan X,Khanna S,Cadenas E,Sen CK

PA5-17831 was used in Western Blotting to observe that safe natural products may target tumor cell mitochondria and sharply lower radiation dosage required for tumor management warrants clinical testing.

Sun Jan 10 00:00:00 EST 2021

Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways.

International journal of molecular sciences

Kurowska P,Mlyczyńska E,Estienne A,Barbe A,Rajska I,Soból K,Poniedziałek-Kempny K,Dupont J,Rak A

PA5-17831 was used in Western Blotting to show that vaspin, by improving in vitro oocyte maturation via MAP3/1 and PRKAA1 kinase pathways, can be a new factor to improve in vitro fertilisation protocols.

Tue Dec 08 00:00:00 EST 2020

Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways.

International journal of molecular sciences

Kurowska P,Mlyczyńska E,Estienne A,Barbe A,Rajska I,Soból K,Poniedziałek-Kempny K,Dupont J,Rak A

Tue Dec 08 00:00:00 EST 2020

Fasting-mimicking diet and hormone therapy induce breast cancer regression.

Nature

Caffa I,Spagnolo V,Vernieri C,Valdemarin F,Becherini P,Wei M,Brandhorst S,Zucal C,Driehuis E,Ferrando L,Piacente F,Tagliafico A,Cilli M,Mastracci L,Vellone VG,Piazza S,Cremonini AL,Gradaschi R,Mantero C,Passalacqua M,Ballestrero A,Zoppoli G,Cea M,Arrighi A,Odetti P,Monacelli F,Salvadori G,Cortellino S,Clevers H,De Braud F,Sukkar SG,Provenzani A,Longo VD,Nencioni A

PA5-17831 was used in Western Blotting to support further clinical studies of a fasting-mimicking diet as an adjuvant to oestrogen therapy in hormone-receptor-positive breast cancer.

Wed Jul 01 00:00:00 EDT 2020

Cryptotanshinone from the Salvia miltiorrhiza Bunge Attenuates Ethanol-Induced Liver Injury by Activation of AMPK/SIRT1 and Nrf2 Signaling Pathways.

International journal of molecular sciences

Nagappan A,Kim JH,Jung DY,Jung MH

Published figure using Phospho-AMPK alpha-1,2 (Thr183, Thr172) polyclonal antibody (Product # PA5-17831) in Western Blot

Mon Dec 30 00:00:00 EST 2019

C‑type natriuretic peptide prevents angiotensin II‑induced atrial connexin 40 and 43 dysregulation by activating AMP‑activated kinase signaling.

Molecular medicine reports

Ding DZ,Jia YN,Zhang B,Guan CM,Zhou S,Li X,Cui X

PA5-17831 was used in Western Blotting to show that C-type natriuretic peptide (CNP) may be therapeutically useful for clinical conditions involving cardiac dysregulation of Cx expression-related diseases.

Sun Dec 01 00:00:00 EST 2019

Trehalose 6-Phosphate Positively Regulates Fatty Acid Synthesis by Stabilizing WRINKLED1.

The Plant cell

Zhai Z,Keereetaweep J,Liu H,Feil R,Lunn JE,Shanklin J

PA5-17831 was used in Western Blotting to indicate that the trehalose 6-phosphate (T6P) sensitivity of SnRK1 in vivo is GRIK1/GRIK2 dependent.

Mon Oct 01 00:00:00 EDT 2018

Gomisin N Alleviates Ethanol-Induced Liver Injury through Ameliorating Lipid Metabolism and Oxidative Stress.

International journal of molecular sciences

Nagappan A,Jung DY,Kim JH,Lee H,Jung MH

Published figure using Phospho-AMPK alpha-1,2 (Thr183, Thr172) polyclonal antibody (Product # PA5-17831) in Western Blot

Sat Sep 01 00:00:00 EDT 2018

Palmitoleic acid reduces intramuscular lipid and restores insulin sensitivity in obese sheep.

Diabetes, metabolic syndrome and obesity : targets and therapy

Duckett SK,Volpi-Lagreca G,Alende M,Long NM

PA5-17831 was used in western blot to investigate the effects of palmitoleic acid on lipogenesis and circulating insulin levels in obese sheep

Thu Nov 27 00:00:00 EST 2014

生物信息学

蛋白别名: 5'-AMP-activated protein kinase alpha-1 catalytic subunit; 5'-AMP-activated protein kinase alpha-2 catalytic subunit; 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; 63 kDa subunit; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase; AMP-activated protein kinase alpha 2; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK 63 kDa subunit; AMPK alpha 1; AMPK alpha 2; AMPK alpha-1 chain; AMPK alpha-2 chain; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK-activated protein kinase alpha-2 subunit; AMPK-alpha-1 chain; AMPK-alpha-2 chain; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; pAMPK; prkaa2; protein kinase; protein kinase AMP-activated alpha 2 catalytic subunit; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; Tau-protein kinase PRKAA1

基因别名: 2310008I11Rik; A830082D05; AI194361; AI450832; AL024255; AMPK; AMPK1; AMPK2; AMPKa1; AMPKA2; AMPKalpha1; AMPKalpha2; C130083N04Rik; PRKAA; PRKAA1; PRKAA2

UniProt ID: (Human) Q13131, (Human) P54646, (Pig) Q28948, (Rat) P54645, (Mouse) Q5EG47, (Rat) Q09137, (Mouse) Q8BRK8

Entrez Gene ID: (Pig) 100145903, (Chicken) 427185, (Human) 5562, (Bovine) 540404, (Bovine) 538954, (Human) 5563, (Pig) 397504, (Chicken) 429110, (Rat) 65248, (Mouse) 105787, (Rat) 78975, (Mouse) 108079