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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • AMPK alpha-1,2 Antibodies

          Invitrogen

          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

          高级验证
          8个已发表图片
          14 篇参考文献
          查看其他15个AMPK alpha-1,2抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

          • 抗体检测数据 (2)
          • 已发表图片 (8)
          • 高级验证 (2)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 12

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          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody (PA5-17831) in WB

          Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), A-431 treated with Metformin (10mM for 24 h) (Lane 2), Hep G2 (Lane 3), Hep G2 treated with Metformin (10mM for 24 h) (Lane 4), U-87 MG (Lane 5), and U-87 MG treated with AICAR (1mM for 48 h) (Lane 6). The blot was probed with Phospho-AMPK alpha-1,2 (Thr183, Thr172) Monoclonal Antibody (Product # PA5-17831, 1:1000 d... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody

          产品信息

          PA5-17831

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000
          查看14篇已发表文章 14篇已发表文章
          产品规格

          种属反应

          Human, Mouse, Non-human primate, Rat

          已发表种属

          Human, Mouse, Pig, Plant, Rat, Sheep

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          Synthetic phosphopeptides corresponding to residues surrounding Thr183 of human AMPKa 1 and Thr172 of AMPKa 2
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          83 µg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/mL BSA

          内含物

          no preservative

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10983715

          产品详细信息

          It is not recommended to aliquot this antibody.

          靶标信息

          The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha 2; AMPK alpha-1 chain; AMPK alpha-2 chain; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK-alpha-1 chain; AMPK-alpha-2 chain; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; pAMPK; prkaa2; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; Tau-protein kinase PRKAA1

          查看更多 收起

          基因别名: 2310008I11Rik; A830082D05; AI194361; AI450832; AL024255; AMPK; AMPK1; AMPK2; AMPKa1; AMPKa2; AMPKalpha1; AMPKalpha2; C130083N04Rik; PRKAA; PRKAA1; PRKAA2

          查看更多 收起

          UniProt ID: (Human) Q13131, (Human) P54646, (Rat) P54645, (Mouse) Q5EG47, (Rat) Q09137, (Mouse) Q8BRK8

          查看更多 收起

          Entrez Gene ID: (Human) 5562, (Human) 5563, (Rat) 65248, (Mouse) 105787, (Rat) 78975, (Mouse) 108079

          查看更多 收起

          功能
          chromatin binding protein kinase activity AMP-activated protein kinase activity cAMP-dependent protein kinase activity protein binding ATP binding protein C-terminus binding kinase binding histone serine kinase activity metal ion binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity tau-protein kinase activity [acetyl-CoA carboxylase] kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity eukaryotic elongation factor-2 kinase activator activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein binding, bridging non-receptor serine/threonine protein kinase
          参与通路
          activation of MAPK activity response to hypoxia glucose metabolic process transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation fatty acid biosynthetic process cholesterol biosynthetic process cell cycle arrest signal transduction positive regulation of cell proliferation lipid biosynthetic process response to UV cold acclimation response to gamma radiation positive regulation of autophagy positive regulation of gene expression response to activity Wnt signaling pathway macroautophagy fatty acid oxidation response to caffeine cellular response to nutrient levels negative regulation of TOR signaling regulation of peptidyl-serine phosphorylation histone-serine phosphorylation intracellular signal transduction cellular response to glucose starvation glucose homeostasis regulation of circadian rhythm negative regulation of apoptotic process positive regulation of cholesterol biosynthetic process positive regulation of glycolytic process negative regulation of glucosylceramide biosynthetic process rhythmic process positive regulation of skeletal muscle tissue development negative regulation of lipid catabolic process protein heterooligomerization fatty acid homeostasis regulation of vesicle-mediated transport cellular response to hydrogen peroxide cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to organonitrogen compound cellular response to hypoxia response to camptothecin regulation of signal transduction by p53 class mediator regulation of energy homeostasis negative regulation of glucose import in response to insulin stimulus carnitine shuttle response to stress response to muscle activity positive regulation of macroautophagy regulation of macroautophagy cellular response to drug regulation of fatty acid biosynthetic process autophagy negative regulation of translation response to hydrogen peroxide positive regulation of gluconeogenesis positive regulation of fatty acid oxidation positive regulation of glucose import lipid metabolic process fatty acid metabolic process steroid biosynthetic process steroid metabolic process cholesterol metabolic process sterol biosynthetic process phosphorylation chromatin modification regulation of lipid metabolic process regulation of gene expression
          It has to be done as per old AB suggested Products section.
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