Invitrogen

Phospho-ATF2 (Thr71) Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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Phospho-ATF2 (Thr71) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-ATF-2 pThr71 showing staining in the nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Phospho-ATF-2 pThr71 Polyclonal Antibody (Product # 44-295G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Phospho-ATF2 (Thr71) Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), U-87 MG treated with 25 µg/mL anisomycin for 30 minutes (Lane 2), K562 (Lane 3), K562 treated with 25 µg/mL anisomycin for 30 minutes (Lane 4). The blots were probed with Anti-Phospho-ATF-2 pThr71 Rabbit Polyclonal Antibody (Product # 44-295G, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A ~ 54 kDa band corresponding to Phospho-ATF-2 pThr71 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Phospho-ATF2 (Thr71) Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Phospho-ATF2 (Thr71) Polyclonal Antibody (Product # 44-295G), shows increased levels of phospho-ATF2 (Thr71) in U-87MG and K562 cell lines upon Anisomycin treatment. {TM}

产品信息

44-295G

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:1,000	-
免疫组化（石蜡） (IHC (P))	1:20	-

产品规格
种属反应	Human
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Chemically synthesized phosphopeptide derived from a region of human ATF2 that contains threonine 71
偶联物	Unconjugated
形式	Liquid
纯化类型	Antigen affinity chromatography
保存液	Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/mL BSA
内含物	0.05% sodium azide
保存条件	-20°C
RRID	AB_2533624

产品详细信息

This antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated ATF2. The final product is generated by affinity chromatography using an ATF2-derived peptide phosphorylated at threonine 71. The peptide sequence is conserved in mouse, rat, chicken and frog. Suggested Western blot positive controls: NIH3T3 + anisomycin, or Jurkat + 10 µg/mL anisomycin for 60 minutes.

靶标信息

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Activating 2; Activating transcription factor 2; activating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; CREB-2; Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; MGC111558; OTTHUMP00000205345; OTTHUMP00000205347; OTTHUMP00000205348; OTTHUMP00000205350; OTTHUMP00000205352; OTTHUMP00000205353; OTTHUMP00000205354

基因别名: ATF2; CRE-BP1; CREB-2; CREB2; CREBP1; HB16; TREB7

UniProt ID: (Human) P15336

Entrez Gene ID: (Human) 1386

分子生物学功能: basic leucine zipper transcription factor

功能

negative regulation of transcription from RNA polymerase II promoter outflow tract morphogenesis regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter response to osmotic stress cellular response to DNA damage stimulus response to water deprivation histone acetylation intra-S DNA damage checkpoint positive regulation of transforming growth factor beta2 production positive regulation of neuron apoptotic process fat cell differentiation positive regulation of transcription from RNA polymerase II promoter negative regulation of epithelial cell proliferation regulation of sequence-specific DNA binding transcription factor activity positive regulation of sequence-specific DNA binding transcription factor activity adipose tissue development amelogenesis positive regulation of mitochondrial membrane permeability involved in apoptotic process

参与通路

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity histone acetyltransferase activity protein binding cAMP response element binding protein binding protein kinase binding cAMP response element binding metal ion binding protein heterodimerization activity

Phospho-ATF2 (Thr71) Polyclonal Antibody

产品信息

44-295G

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

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