Invitrogen

Phospho-Bora (Ser497) Polyclonal Antibody

产品信息

PA5-64657

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:1,000	-

产品规格
种属反应	Human, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	A synthesized peptide derived from human BORA (Phospho-Ser497) 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.4, with 50% glycerol
内含物	0.02% sodium azide
保存条件	-20°C
RRID	AB_2663531

产品详细信息

Phospho-Bora (Ser497) Polyclonal Antibody detects endogenous levels of Bora only when phosphorylated at Ser497.

靶标信息

Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

仅用于科研。不用于诊断过程。未经明确授权不得转售。