Invitrogen

Phospho-CaMKII (Thr305,Thr306) Recombinant Rabbit Monoclonal Antibody (9H2L7)

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Phospho-CaMKII (Thr305,Thr306) Antibody in Immunocytochemistry (ICC/IF) — For immunofluorescence analysis, SH-SY5Y cells were fixed and permeabilized for detection of endogenous CamKIIpT305/306 using Anti- CamKIIpT305/306 Recombinant Rabbit Monoclonal Antibody (Product # 702357, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CamKIIpT305/306 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of CamKIIpT305/306. Panel e) shows loss of signal by competition with the CamKIIpT305/306 peptide, demonstrating antibody specificity. Panel f) demonstrates no competition with the non-phosphorylated peptide. Panel g) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Phospho-CaMKII (Thr305,Thr306) Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), SH-SY5Y (Lane 2) and Neuro-2a (Lane 3). The blots were probed with Anti-CamKII p (T305/306) Recombinant Rabbit Monoclonal Antibody (Product # 702357, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 63 kDa band corresponding to CamKII p (T305/306) was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

产品信息

702357

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1-2 µg/mL	-
免疫细胞化学 (ICC/IF)	2 µg/mL	-

产品规格
种属反应	Human
宿主/亚型	Rabbit / IgG
分类	Recombinant Monoclonal
类型	Antibody
克隆号	9H2L7
抗原	Peptide corresponding to Human CMK2A (aa 300-309)
偶联物	Unconjugated
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Protein A
保存液	PBS, pH 7.2
内含物	0.09% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2632984

产品详细信息

This antibody is predicted to react with Monkey, Mouse, Dog, Sheep and Bovine

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+/calmodulin (CaM) binding and autophosphorylation and has been implicated in regulation of the cell cycle and transcription. It is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Calci; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaM-kinase II alpha chain; CaMK-II alpha subunit; caMK-II subunit alpha; CaMKIINalpha; EC 2.7.11.17; OTTHUMP00000165787; OTTHUMP00000165788; Phospho-CaMKI

基因别名: CAMK2A; CAMKA; KIAA0968

UniProt ID: (Human) Q9UQM7

Entrez Gene ID: (Human) 815

分子生物学功能: non-receptor serine/threonine protein kinase

功能

G1/S transition of mitotic cell cycle MAPK cascade response to ischemia protein phosphorylation calcium ion transport Wnt signaling pathway, calcium modulating pathway positive regulation of cardiac muscle cell apoptotic process peptidyl-serine phosphorylation angiotensin-activated signaling pathway positive regulation of GTPase activity protein autophosphorylation regulation of neurotransmitter secretion regulation of neuronal synaptic plasticity positive regulation of NF-kappaB transcription factor activity positive regulation of calcium ion transport interferon-gamma-mediated signaling pathway regulation of cellular response to heat regulation of mitochondrial membrane permeability involved in apoptotic process

参与通路

protein serine/threonine kinase activity calmodulin-dependent protein kinase activity Ras guanyl-nucleotide exchange factor activity protein binding calmodulin binding ATP binding kinase activity glutamate receptor binding protein homodimerization activity

Phospho-CaMKII (Thr305,Thr306) Recombinant Rabbit Monoclonal Antibody (9H2L7)

产品信息

702357

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

种属反应

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

篇参考文献 (0)

生物信息学

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