Invitrogen

Phospho-CaMKIV (Thr196, Thr200) Polyclonal Antibody

Product Image Gallery

Phospho-CaMKIV (Thr196, Thr200) Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of Phospho-CaMKIV (Thr196, Thr200) in Hela cells, using Phospho-CaMKIV (Thr196, Thr200) Polyclonal Antibody (Product # PA5-37504). The picture on the right is blocked with the phospho peptide.

Phospho-CaMKIV (Thr196, Thr200) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Phospho-CaMKIV (Thr196, Thr200) in paraffin-embedded Human brain tissue using Phospho-CaMKIV (Thr196, Thr200) Polyclonal Antibody (Product # PA5-37504) (left). The picture on the right is blocked with the pospho peptide.

产品信息

PA5-37504

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:1,000	查看2篇已发表文章 2篇已发表文章
免疫组化（石蜡） (IHC (P))	Assay-Dependent	-
免疫细胞化学 (ICC/IF)	Assay-Dependent	-

产品规格
种属反应	Human, Mouse, Rat
已发表种属	Mouse, Rhesus monkey
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Peptide sequence around phosphorylation site of threonine 200 (M-K-T(p)-V-C) derived from Human CaMK4 or threonine 196 (M-K-T(p)-V-C) derived from Mouse CaMK4. 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.4, with 50% glycerol
内含物	0.02% sodium azide
保存条件	-20°C
RRID	AB_2554113

产品详细信息

A suggested positive control for Western blot is K562 cells.

靶标信息

CaM kinase IV (CaMKIV), in a calcium and calmodulin dependent manner, is implicated in phosphorylating numerous transcription factors including the CCAAT enhancer binding protein (C/EBP), serum response factor (SRF), and cyclic AMP response element binding protein (CBP). The nuclear localization of this protein is consistent with its role in mediating calcium-dependent gene expression. CaMKIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaMKIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T-cells (NFAT). CaMKIV is encoded, along with calspermin, by the CaMKIV gene. It has been found that, in testes, CaMKIV is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SIV-Mediated Synaptic Dysfunction Is Associated with an Increase in Synapsin Site 1 Phosphorylation and Impaired PP2A Activity.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Shekarabi M,Robinson JA,Smith MD,Burdo TH

PA5-37504 was used in Western Blotting to show that during SIV infection increased phosphorylation of Syn I serine 9, site 1, and lower protein phosphatase 2A (PP2A) activity supports the occurrence of specific impairments in synaptic activity.

Wed Aug 28 00:00:00 EDT 2019

Ca²⁺-Dependent Regulation of NFATc1 via KCa3.1 in Inflammatory Osteoclastogenesis.

Journal of immunology (Baltimore, Md. : 1950)

Grössinger EM,Kang M,Bouchareychas L,Sarin R,Haudenschild DR,Borodinsky LN,Adamopoulos IE

PA5-37504 was used in Western Blotting to evaluate KCa3.1 as a modulator of Ca2+-induced NFAT-dependent osteoclast differentiation in inflammatory bone loss.

Mon Jan 15 00:00:00 EST 2018

生物信息学

蛋白别名: brain Ca(2+)-calmodulin-dependent protein kinase type IV; brain Ca++-calmodulin-dependent protein kinase type IV; Ca2+/calmodulin-dependent protein kinase type IV/Gr; Calcium/calmoduli; calcium/calmodulin-dependent protein kinase IV; Calcium/calmodulin-dependent protein kinase type IV; calcium/calmodulin-dependent protein kinase type IV catalytic chain; Calmodulin-dependent protein kinase IV; Calspermin; CAM kinase IV; CAM kinase- GR; CAM kinase-GR; CaMK IV; EC 2.7.11.17; KCC4; kinase CaMK4; OTTHUMP00000222818

基因别名: A430110E23Rik; AI666733; CAMK; CaMK IV; CAMK-GR; CAMK4; CAMKIV; CaMKIV/Gr; Ccdpk; D18Bwg0362e; RATCCDPK

UniProt ID: (Human) Q16566, (Rat) P13234

Entrez Gene ID: (Human) 814, (Mouse) 12326, (Rat) 25050

分子生物学功能: non-receptor serine/threonine protein kinase