Invitrogen

Phospho-CrkL (Tyr207) Recombinant Rabbit Monoclonal Antibody (4H24L33)

Phospho-CrkL (Tyr207) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-CrkL (Tyr207) Antibody (702815) in ICC/IF

For immunofluorescence analysis, K562 cells were fixed and unpermeabilized for detection of endogenous CrKL pY207 using Anti-CrKL pY207 Recombinant Rabbit Monoclonal Antibody (Product # 702815, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) is stained using SlowFade® Gold Antifade Mount... View More

Phospho-CrkL (Tyr207) Antibody in Western Blot (WB)

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产品信息

702815

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

4H24L33

抗原

Peptide corresponding to human phospho-CRKL (Tyr207) [aa204-aa210]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2734824

产品详细信息

This antibody is predicted to react with Monkey, Cat, Horse, Mouse.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

The CRKL gene encodes the CRK-like protein, an adaptor protein that plays a crucial role in intracellular signaling pathways related to cell proliferation, differentiation, and migration. CRKL contains SH2 and SH3 domains that facilitate interactions with other signaling molecules, influencing multiple pathways such as the MAPK, PI3K, and STAT pathways. It is involved in processes like cytoskeleton reorganization and immune cell signaling, with significant implications for development and cancer. CRKL is notably associated with Philadelphia chromosome-positive leukemias, where it interacts with the BCR-ABL fusion protein, contributing to leukemogenesis. Additionally, dysregulation of CRKL can affect cardiovascular development, leading to congenital heart defects. Research into CRKL focuses on understanding its role in signal transduction and its potential as a therapeutic target due to its involvement in oncogenic and developmental pathways.

仅用于科研。不用于诊断过程。未经明确授权不得转售。