Invitrogen

Phospho-CrkL (Tyr207) Recombinant Rabbit Monoclonal Antibody (CrkLY207-G4)

查看其他27个CrkL抗体

抗体检测数据 (6)

Phospho-CrkL (Tyr207) Antibody in Western Blot (WB)

Phospho-CrkL (Tyr207) Antibody in Western Blot (WB)

图: 1 / 6

Phospho-CrkL (Tyr207) Antibody (MA5-36969) in WB

Western blot analysis of Phospho-CrkL (Tyr207) using mouse wild-type (+/+) or Crkl knock out (-/-) whole embryos (loading control, red). Samples were incubated in monoclonal Phospho-CrkL (Tyr207) antibody (Product # MA5-36969) with a dilution of 1:500.

Phospho-CrkL (Tyr207) Antibody in Western Blot (WB)

Phospho-CrkL (Tyr207) Antibody in Western Blot (WB)

Phospho-CrkL (Tyr207) Antibody in Western Blot (WB)

Phospho-CrkL (Tyr207) Antibody in Flow Cytometry (Flow)

Phospho-CrkL (Tyr207) Antibody in Flow Cytometry (Flow)

Phospho-CrkL (Tyr207) Antibody in Flow Cytometry (Flow)

Product Image Gallery

Phospho-CrkL (Tyr207) Antibody in Western Blot (WB) — Western blot analysis of Phospho-CrkL (Tyr207) using mouse wild-type (+/+) or Crkl knock out (-/-) whole embryos (loading control, red). Samples were incubated in monoclonal Phospho-CrkL (Tyr207) antibody (Product # MA5-36969) with a dilution of 1:500.

Phospho-CrkL (Tyr207) Antibody in Western Blot (WB) — Western blot analysis of Phospho-CrkL (Tyr207) using K562 cell extract. Samples were untreated or treated with imatinib, and incubated in monoclonal Phospho-CrkL (Tyr207) antibody (Product # MA5-36969) with a dilution of 1 ng/mL.

Phospho-CrkL (Tyr207) Antibody in Western Blot (WB) — Western blot analysis of Phospho-CrkL (Tyr207) using NIH3T3 cell extract. Samples were incubated in monoclonal Phospho-CrkL (Tyr207) antibody (Product # MA5-36969) with a dilution of 1 µg/mL.

Phospho-CrkL (Tyr207) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-CrkL (Tyr207) using K562 cells. Samples were incubated in monoclonal Phospho-CrkL (Tyr207) antibody (Product # MA5-36969) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (light blue), treated with imatinib (red), treated with pervanadate (green), imatinib and blocked with phospho-peptide (black), pervanadate and blocked with phospho peptide (gold), imatinib and blocked with non-phospho peptide (dark blue), pervanadate and blocked with non-phospho peptide (purple).

Phospho-CrkL (Tyr207) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-CrkL (Tyr207) using K562 cells. Samples were incubated in monoclonal Phospho-CrkL (Tyr207) antibody (Product # MA5-36969) with a dilution of 0.05 µg/mL. Images correspond with the following conditions: negative control (blue), treated with imatinib (red), with pervanadate (green).

Phospho-CrkL (Tyr207) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-CrkL (Tyr207) using K562 cells. Samples were incubated in monoclonal Phospho-CrkL (Tyr207) antibody (Product # MA5-36969) with a dilution of 0.05 µg/mL. Images correspond with the following conditions: negative control (blue), imatinib (red), treated with pervanadate (green).

产品信息

MA5-36969

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

-

流式细胞分析 (Flow)

1-1,000 ng/mL

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

CrkLY207-G4

抗原

A synthetic phospho-peptide corresponding to residues surrounding Tyr207 of human phospho CrkL.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 50% glycerol, 0.10% BSA

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2896904

靶标信息

May mediate the transduction of intracellular signals.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Crk-like protein; OTTHUMP00000198539; v-crk avian sarcoma virus CT10 oncogene homolog-like

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基因别名: 1110025F07Rik; AA589403; AI325100; CRKL; Crkol

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UniProt ID: (Human) P46109, (Mouse) Q8BGC5

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Entrez Gene ID: (Human) 1399, (Mouse) 12929

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signal transducer activity SH3/SH2 adaptor activity protein binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion scaffold/adaptor protein

参与通路

activation of MAPKK activity blood vessel development JNK cascade Ras protein signal transduction heart development positive regulation of cell proliferation organ morphogenesis anterior/posterior pattern specification intracellular signal transduction thymus development parathyroid gland development cell-cell adhesion positive regulation of substrate adhesion-dependent cell spreading pattern specification process