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Phospho-DENND3 (Ser554) Antibody (PA5-95668) in WB
Western blot of DENND3 in HeLa cell lysate showing specific immunolabeling of a band at ~142 kDa corresponding to Phospho-DENND3 (Ser554) polyclonal antibody (Product # PA5-95668) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (800 units/1 mg protein for 30 min).
Product Details
PA5-95668
产品规格
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser554 conjugated to KLH
Conjugate
Unconjugated
形式
liquid
浓度
0.25 mg/mL
纯化类型
Antigen Affinity Chromatography
保存液
10mM HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 0.15M NaCl
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2807470
Product Specific Information
Antigen Affinity Purified from Pooled Serum
Target Information
Guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (PubMed:20937701). Regulates autophagy in response to starvation through RAB12 activation. Starvation leads to ULK1/2-dependent phosphorylation of Ser-472 and Ser-490, which in turn allows recruitment of 14-3-3 adapter proteins and leads to up-regulation of GEF activity towards RAB12. Also plays a role in protein transport from recycling endosomes to lysosomes, regulating, for instance, the degradation of the transferrin receptor and of the amino acid transporter PAT4 (PubMed:20937701). Starvation also induces phosphorylation at Tyr-858, which leads to up-regulated GEF activity and initiates autophagy. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: DENN domain-containing protein 3; DENN/MADD domain containing 3
Gene Aliases: DENND3; KIAA0870
UniProt ID: (Human) A2RUS2
Entrez Gene ID: (Human) 22898
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