Invitrogen

Phospho-ERK1/ERK2 (Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (15H10L7)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4个已发表图片

13 篇参考文献

查看其他62个ERK1/2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (700012) in ICC/IF

Immunofluorescence analysis of Phosph-ERK1/2 (Thr 185,Tyr187) was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ERK2 Rabbit monoclonal Antibody (Product # 700012) at 5 µg/mL in 0.1% BSA and incubated over... View More

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Western Blot (WB)

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody in Immunohistochemistry (IHC)

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody

Product Image Gallery

产品信息

700012

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看10篇已发表文章 10篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

酶联免疫吸附实验 (ELISA)

Assay-dependent

染色质免疫沉淀实验 (ChIP)

1 µL

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Dog, Human, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

15H10L7

抗原

A peptide corresponding to amino acids 198-208 and 181-191 of P27361 and P28482, respectively.

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532267

产品详细信息

700012 has successfully been used in western blot to detect phospho-ERK1/2 in TPA-treated HeLa, NRK and MDCK cell lysates.

This antibody is predicted to react with bovine, chicken, chimpanzee, mouse, rat, Xenopus and zebrafish based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Dasabuvir suppresses esophageal squamous cell carcinoma growth in vitro and in vivo through targeting ROCK1.

Cell death & disease

Liu X,Jiang Y,Zhou H,Zhao X,Li M,Bao Z,Wang Z,Zhang C,Xie Z,Zhao J,Dong Z,Liu K,Guo Z

700012 was used in ChIP Assay, ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to provide evidence that dasabuvir suppresses ESCC growth in vivo and in vitro through blocking ROCK1/ERK signaling pathway.

Mon Feb 13 00:00:00 EST 2023

Temporal activation of WNT/β-catenin signaling is sufficient to inhibit SOX10 expression and block melanoma growth.

Oncogene

Uka R,Britschgi C,Krättli A,Matter C,Mihic D,Okoniewski MJ,Gualandi M,Stupp R,Cinelli P,Dummer R,Levesque MP,Shakhova O

700012 was used in Immunohistochemistry-immunofluorescence to show that MEK and RAF inhibitors do not suppress levels of SOX10 protein in patient-derived cells in vitro, as well as in melanoma patients in vivo.

Fri May 01 00:00:00 EDT 2020

Temporal activation of WNT/β-catenin signaling is sufficient to inhibit SOX10 expression and block melanoma growth.

Oncogene

Uka R,Britschgi C,Krättli A,Matter C,Mihic D,Okoniewski MJ,Gualandi M,Stupp R,Cinelli P,Dummer R,Levesque MP,Shakhova O

Fri May 01 00:00:00 EDT 2020

HER2-Targeted PET Imaging and Therapy of Hyaluronan-Masked HER2-Overexpressing Breast Cancer.

Molecular pharmaceutics

Pereira PMR,Ragupathi A,Shmuel S,Mandleywala K,Viola NT,Lewis JS

700012 was used in Western Blotting to investigate whether degradation of hyaluronan can resensitize HER2-overexpressing breast cancer cells to trastuzumab.

Mon Jan 06 00:00:00 EST 2020

LQB‑118 compound inhibits migration and induces cell death in glioblastoma cells.

Oncology reports

Bernardo PS,Guimarães GHC,De Faria FCC,Longo GMDC,Lopes GPF,Netto CD,Costa PRR,Maia RC

700012 was used in Western Blotting to demonstrate that LQB‚118 maintained its effectiveness in a 3D cell conformation, which shares more similarities with the tumor mass.

Wed Jan 01 00:00:00 EST 2020

Alterations of the renin angiotensin system in human end-stage heart failure before and after mechanical cardiac unloading by LVAD support.

Molecular and cellular biochemistry

Messmann R,Dietl A,Wagner S,Domenig O,Jungbauer C,Luchner A,Maier LS,Schopka S,Hirt S,Schmid C,Birner C

700012 was used in Western Blotting to determine the regulation patterns of key components of the RAS and the β-arrestin signaling pathways in left ventricular (LV) tissue.

Tue Sep 01 00:00:00 EDT 2020

Disc1 Carrier Mice Exhibit Alterations in Neural pIGF-1Rβ and Related Kinase Expression.

Frontiers in cellular neuroscience

Sultana R,Shrestha A,Lee CC,Ogundele OM

700012 was used in Western Blotting to demonstrate that the expression of active IGF-1Rβ (pIGF-1Rβ) is altered in the hippocampus and prefrontal cortex (PFC) of disc1 mutant mice and vary with the dose of the mutation (homozygous and heterozygous).

Mon Sep 28 00:00:00 EDT 2020

Disc1 Carrier Mice Exhibit Alterations in Neural pIGF-1Rβ and Related Kinase Expression.

Frontiers in cellular neuroscience

Sultana R,Shrestha A,Lee CC,Ogundele OM

Mon Sep 28 00:00:00 EDT 2020

SK Channel Modulates Synaptic Plasticity by Tuning CaMKIIα/β Dynamics.

Frontiers in synaptic neuroscience

Shrestha A,Sultana R,Lee CC,Ogundele OM

700012 was used in Western Blotting to demonstrate that positive modulation of SK channel at hippocampal synapses likely refine GluN1-linked plasticity by tuning dendritic localization of CaMKIIα.

Tue Sep 29 00:00:00 EDT 2020

Neonatal maternal deprivation impairs localized de novo activity-induced protein translation at the synapse in the rat hippocampus.

Bioscience reports

Ahmad F,Salahuddin M,Alsamman K,Herzallah HK,Al-Otaibi ST

700012 was used in Western Blotting to elucidate the molecular mechanisms underlying the detrimental alterations in brain functions induced by early-life stress.

Fri Jun 29 00:00:00 EDT 2018

Metformin exerts multitarget antileukemia activity in JAK2^V617F-positive myeloproliferative neoplasms.

Cell death & disease

Machado-Neto JA,Fenerich BA,Scopim-Ribeiro R,Eide CA,Coelho-Silva JL,Dechandt CRP,Fernandes JC,Rodrigues Alves APN,Scheucher PS,Simões BP,Alberici LC,de Figueiredo Pontes LL,Tognon CE,Druker BJ,Rego EM,Traina F

Published figure using Phospho-ERK1/ERK2 (Thr185, Tyr187) recombinant monoclonal antibody (Product # 700012) in Western Blot

Thu Feb 22 00:00:00 EST 2018

Integrator orchestrates RAS/ERK1/2 signaling transcriptional programs.

Genes & development

Yue J,Lai F,Beckedorff F,Zhang A,Pastori C,Shiekhattar R

700012 was used in Chromatin immunoprecipitation to demonstrate that the MAPK transcriptional response in human cells is funneled through Integrator, an RNA polymerase II-associated complex.

Fri Sep 01 00:00:00 EDT 2017

ANKHD1 silencing inhibits Stathmin 1 activity, cell proliferation and migration of leukemia cells.

Biochimica et biophysica acta

Machado-Neto JA,Lazarini M,Favaro P,de Melo Campos P,Scopim-Ribeiro R,Franchi Junior GC,Nowill AE,Lima PR,Costa FF,Benichou S,Olalla Saad ST,Traina F

700012 was used in western blot to identify binding partners ANKHD1 in leukemia cells

Sun Mar 01 00:00:00 EST 2015

TGF-β mediates early angiogenesis and latent fibrosis in an Emilin1-deficient mouse model of aortic valve disease.

Disease models & mechanisms

Munjal C,Opoka AM,Osinska H,James JF,Bressan GM,Hinton RB

700012 was used in Immunohistochemistry to show that manifestation of EFF and aberrant angiogenesis suggests they are crucial intermediate factors involved in aortic valve disease.

Fri Aug 01 00:00:00 EDT 2014

Multidimensional profiling of CSF1R screening hits and inhibitors: assessing cellular activity, target residence time, and selectivity in a higher throughput way.

Journal of biomolecular screening

Uitdehaag JC,Sünnen CM,van Doornmalen AM,de Rouw N,Oubrie A,Azevedo R,Ziebell M,Nickbarg E,Karstens WJ,Ruygrok S

700012 was used in western blot to develop medium throughput assays to characterize CSF1R HTS hits and aid the selection of the best compounds for lead progression

Sat Oct 01 00:00:00 EDT 2011

生物信息学

蛋白别名: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42 MAP Kinase; p42-MAPK; p44-ERK1; p44-MAPK; protein tyrosine kinase ERK2

查看更多收起

基因别名: ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

查看更多收起

功能

phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding scaffold protein binding protein kinase activity protein kinase binding non-receptor serine/threonine protein kinase

参与通路

Phospho-ERK1/ERK2 (Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (15H10L7)

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (700012) in ICC/IF

产品信息

700012

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

Expression System

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

Phospho-ERK1/ERK2 (Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (15H10L7)

Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (700012) in ICC/IF

产品信息

700012

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

Expression System

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)