Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
1 µg/mL | 查看8篇已发表文章 8篇已发表文章 |
免疫组化 (IHC) |
- | 查看2篇已发表文章 2篇已发表文章 |
免疫组化(石蜡) (IHC (P)) |
5 µg/mL | - |
免疫细胞化学 (ICC/IF) |
5 µg/mL | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
染色质免疫沉淀 实验 (ChIP) |
1 µL | 查看1篇已发表文章 1篇已发表文章 |
产品规格 | |
---|---|
种属反应 |
Dog, Human, Rat |
已发表种属 |
Human, Mouse, Rat |
宿主/亚型 |
Rabbit / IgG |
分类 |
Recombinant Monoclonal |
类型 |
Antibody |
克隆号 |
15H10L7 |
抗原 |
A peptide corresponding to amino acids 198-208 and 181-191 of P27361 and P28482, respectively. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
0.5 mg/mL |
纯化类型 |
Protein A |
保存液 |
PBS |
内含物 |
0.09% sodium azide |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2532267 |
700012 has successfully been used in western blot to detect phospho-ERK1/2 in TPA-treated HeLa, NRK and MDCK cell lysates.
This antibody is predicted to react with bovine, chicken, chimpanzee, mouse, rat, Xenopus and zebrafish based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42 MAP Kinase; p42-MAPK; p44-ERK1; p44-MAPK; protein tyrosine kinase ERK2
基因别名: ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3
UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Rat) P21708
Entrez Gene ID: (Human) 5594, (Dog) 477575, (Human) 5595, (Dog) 100855877, (Rat) 116590, (Rat) 50689