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Invitrogen
Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody
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Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody (PA1-4607) in ICC/IF
Immunofluorescent analysis of Phospho-MAPK1/3 pThr202/Tyr204 (green) in HeLa cells either left untreated (left panel) or treated with 50nM PMA (right panel) for 10 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-MAPK1/3 pThr2... View More
产品信息
PA1-4607
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 of rat MAPK1/2 conjugated to KLH
偶联物
形式
Liquid
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primate, Xenopus and zebrafish based on 100% sequence homology.
In Western blot, this antibody detects an ~42-44 kDa protein representing Phospho-Thr202/Thr204 ERK/MAPK.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. Further optimization, including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody, may have to be performed for your high content assay.
靶标信息
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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