Invitrogen

Phospho-EpoR (Tyr454, Tyr456) Recombinant Superclonal Antibody (3HCLC)

Phospho-EpoR (Tyr454, Tyr456) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

Phospho-EpoR (Tyr454, Tyr456) Antibody (710484) in ICC/IF

Immunofluorescent analysis of EPO Receptor (pYpY454/456) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with EPO Receptor (pYpY454/456) Recombinant Rabbit Polyclonal Antibody (Product # 710484) at a dilution of 1:5... View More

Phospho-EpoR (Tyr454, Tyr456) Antibody in Western Blot (WB)

Product Image Gallery

产品信息

710484

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

克隆号

3HCLC

抗原

Phosphorylation of the human EPO Receptor at the Tyr454 and Tyr456

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532724

产品详细信息

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

靶标信息

EpoR encodes the erythropoietin receptor, which belongs to the cytokine receptor family. When erythropoietin binds to this receptor, it activates the Jak2 tyrosine kinase, which in turn activates various intracellular pathways including Ras/MAP kinase, phosphatidylinositol 3-kinase, and STAT transcription factors. The stimulated erythropoietin receptor plays a role in the survival of erythroid cells. Defects in this gene may result in erythroleukemia and familial erythrocytosis. Dysregulation of this gene may also affect the growth of certain tumors. Multiple transcript variants are produced as a result of alternate splicing. Diseases associated with EPOR include Erythrocytosis, Familial, 1 and Erythroleukemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。