Invitrogen

Phospho-FAK (Tyr576) Recombinant Rabbit Monoclonal Antibody (2H74L24)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8个已发表图片

9 篇参考文献

查看其他49个FAK抗体

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Phospho-FAK (Tyr576) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Phospho-FAK (Tyr576) Antibody was performed using 90% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-FAK (Tyr576) Recombinant Rabbit Monoclonal Antibody (Product # 700013) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Recombinant Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows untreated cells with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Phospho-FAK (Tyr576) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FAK showing staining in the cytoplasm of paraffin-embedded human cervices carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a FAK monoclonal antibody (Product # 700013) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Phospho-FAK (Tyr576) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Figure 3 FRNK does not act as a dominant negative in early Xenopus embryos. (A-D) Optical sections of whole mount immunostained embryos injected with 1 ng GFP-FRNK at the two dorsal blastomeres at the four-cell stage. Embryos were stained with anti-GFP (A) and anti-P-Y397 (B). C is the merged image and D an intensity color coded image of the anti-P-Y397 signal. FRNK injected cells are indicated with red stars and control cells with white stars. FRNK expression fails to reduce the phosphorylation levels of endogenous FAK on tyrosine 397. (E-H) Same as A-D, but the embryos were stained with anti-GFP (E) and anti-P-Y576 (F). FRNK expressing cells display similar levels of phosphorylation on tyrosine 576 as neighboring control cells. (I-L) Confocal images of A6 Xenopus cells transfected with GFP-FRNK. Cells were stained with anti-GFP (I) and anti-P-Y397 (J). K is the merged image and L an intensity color coded image of the anti-P-Y397 signal. FRNK expression leads to reduction of the phosphorylation levels of FAK on tyrosine 397 at the focal adhesions. (M-P) Same as I-L but the cells were stained with anti-GFP (M) and anti-P-Y576 (N) antibodies. FRNK expression leads to downregulation of the endogenous phosphorylation levels of FAK on tyrosine 576 at the focal adhesions. (Q) Western blot analysis of control and injected gastrula stage embryos with 1 ng FRNK at the animal pole of both blastomeres of two cell stage embryos. FRNK expression fails to reduce endogenous FAK phosphoryla

Phospho-FAK (Tyr576) Antibody in Western Blot (WB) — Published figure using Phospho-FAK (Tyr576) recombinant monoclonal antibody (Product # 700013) in Western Blot

Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of Phospho-FAK (Tyr576) using Phospho-FAK (Tyr576) Antibody (2H74L24) (Product # 700013) shows induction of FAK phosphorylation at Tyr576 residue in A-431 cell line upon EGF treatment. {TM}

产品信息

700013

应用	建议稀释比	已发表文章
免疫印迹 (WB)	-	查看5篇已发表文章 5篇已发表文章
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	1:10-1:100	-
免疫细胞化学 (ICC/IF)	1:100	查看2篇已发表文章 2篇已发表文章
其他PubMed (Misc)	-	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human
已发表种属	Human, Mouse, Xenopus
宿主/亚型	Rabbit / IgG
分类	Recombinant Monoclonal
类型	Antibody
克隆号	2H74L24
抗原	A peptide corresponding to amino acids 573-582 of Q05397. 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Protein A
保存液	PBS
内含物	0.09% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2532268

产品详细信息

This antibody is predicted to react with chimpanzee, Rhesus monkey, mouse, rat, chicken, Xenopus, zebrafish and Macaque based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK, and involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase, and activates cell migration and invasion. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

MARCKS Is Necessary for Netrin-DCC Signaling and Corpus Callosum Formation.

Molecular neurobiology

Brudvig JJ,Cain JT,Schmidt-Grimminger GG,Stumpo DJ,Roux KJ,Blackshear PJ,Weimer JM

Published figure using Phospho-FAK (Tyr576) recombinant monoclonal antibody (Product # 700013) in Western Blot

Thu Nov 01 00:00:00 EDT 2018

A ligand-independent integrin β1 mechanosensory complex guides spindle orientation.

Nature communications

Petridou NI,Skourides PA

700013 was used in immunocytochemistry to elucidate how spindle orientation occurs through a ligand-independent intgrin beta 1 mechanosensory complex

Tue Mar 08 00:00:00 EST 2016

Targeting the insulin-like growth factor receptor and Src signaling network for the treatment of non-small cell lung cancer.

Molecular cancer

Min HY,Yun HJ,Lee JS,Lee HJ,Cho J,Jang HJ,Park SH,Liu D,Oh SH,Lee JJ,Wistuba II,Lee HY

700013 was used in western blot to study mechanisms that confer resistance to therapeutic interventions in the insulin-like growth factor receptor pathway

Thu Jun 04 00:00:00 EDT 2015

Understanding the roles of FAK in cancer: inhibitors, genetic models, and new insights.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Yoon H,Dehart JP,Murphy JM,Lim ST

700013 was used in Immunohistochemistry to discuss FAK inhibitors as well as genetic mouse models to provide mechanistic insights into FAK signaling and its potential in cancer therapy.

Sun Feb 01 00:00:00 EST 2015

Understanding the roles of FAK in cancer: inhibitors, genetic models, and new insights.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Yoon H,Dehart JP,Murphy JM,Lim ST

700013 was used in Immunohistochemistry to discuss FAK inhibitors as well as genetic mouse models to provide mechanistic insights into FAK signaling and its potential in cancer therapy.

Sun Feb 01 00:00:00 EST 2015

FAK transduces extracellular forces that orient the mitotic spindle and control tissue morphogenesis.

Nature communications

Petridou NI,Skourides PA

700013 was used in Western Blotting to suggest that FAK transduces external forces to the cell cortex.

Fri Oct 24 00:00:00 EDT 2014

Post-ovulatory aging of oocytes disrupts kinase signaling pathways and lysosome biogenesis.

Molecular reproduction and development

McGinnis LK,Pelech S,Kinsey WH

700013 was used in western blot to assess broad patterns of signaling pathway activity during in vitro oocyte aging.

Wed Oct 01 00:00:00 EDT 2014

Activation of endogenous FAK via expression of its amino terminal domain in Xenopus embryos.

PloS one

Petridou NI,Stylianou P,Christodoulou N,Rhoads D,Guan JL,Skourides PA

Thu Jan 10 00:00:00 EST 2013

Activation of endogenous FAK via expression of its amino terminal domain in Xenopus embryos.

PloS one

Petridou NI,Stylianou P,Christodoulou N,Rhoads D,Guan JL,Skourides PA

Thu Jan 10 00:00:00 EST 2013

生物信息学

蛋白别名: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2

基因别名: FADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71; PTK2

UniProt ID: (Human) Q05397

Entrez Gene ID: (Human) 5747

功能

参与通路

actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding JUN kinase binding protein kinase binding SH2 domain binding

Phospho-FAK (Tyr576) Recombinant Rabbit Monoclonal Antibody (2H74L24)

产品信息

700013

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

其他PubMed (Misc)

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生物信息学

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