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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • FAK Antibodies

          Invitrogen

          Phospho-FAK (Tyr861) Polyclonal Antibody

          高级验证
          41个已发表图片
          67 篇参考文献
          查看其他61个FAK抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite Phospho-FAK (Tyr861) Polyclonal Antibody

          • 抗体检测数据 (4)
          • 已发表图片 (41)
          • 高级验证 (1)
          Phospho-FAK (Tyr861) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-FAK (Tyr861) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 46

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          Phospho-FAK (Tyr861) Antibody (44-626G) in ICC/IF

          Immunofluorescence analysis of Phospho-FAK (Tyr861) was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-FAK (Tyr861) Rabbit Polyclonal Antibody (Product # 44-626G) at 1:250 dilution in 1% BSA and incubated... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          Phospho-FAK (Tyr861) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-FAK (Tyr861) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody
          Phospho-FAK (Tyr861) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-FAK (Tyr861) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Phospho-FAK (Tyr861) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Phospho-FAK (Tyr861) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Phospho-FAK (Tyr861) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Phospho-FAK (Tyr861) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-FAK (Tyr861) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Phospho-FAK (Tyr861) Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (Paraffin) (WB, ICC/IF, IHC (P))
          Phospho-FAK (Tyr861) Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))
          Phospho-FAK (Tyr861) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-FAK (Tyr861) Antibody in Immunohistochemistry (IHC)
          Phospho-FAK (Tyr861) Antibody in Immunohistochemistry (IHC)
          Phospho-FAK (Tyr861) Antibody in Immunohistochemistry (IHC)
          Phospho-FAK (Tyr861) Antibody in Immunohistochemistry (IHC)
          Phospho-FAK (Tyr861) Antibody in Immunohistochemistry (IHC)
          Phospho-FAK (Tyr861) Antibody in Immunohistochemistry (IHC)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr861) Antibody

          产品信息

          44-626G

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000
          查看35篇已发表文章 35篇已发表文章

          免疫组化 (IHC)

          Assay-dependent
          查看13篇已发表文章 13篇已发表文章

          免疫组化(石蜡) (IHC (P))

          -
          查看5篇已发表文章 5篇已发表文章

          免疫细胞化学 (ICC/IF)

          1:250
          查看11篇已发表文章 11篇已发表文章

          免疫沉淀 (IP)

          -
          查看2篇已发表文章 2篇已发表文章

          其他PubMed (Misc)

          -
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Chicken, Human, Mouse

          已发表种属

          Chicken, Dog, Hamster, Human, Mouse, Non-human primate, Rat, Xenopus, Zebrafish

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 861. The sequence is conserved in mouse, rat, chicken and frog.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          纯化类型

          Affinity chromatography

          保存液

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          内含物

          0.05% sodium azide

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533703

          产品详细信息

          The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated FAK. The final product is generated by affinity chromatography using a FAK-derived peptide that is phosphorylated at tyrosine 861.

          Primary chicken embryo fibroblasts (CEF) expressing FAK protein and plated on fibronectin, or A459 whole cell lysate was used as positive control.

          The target antigen, in western blotting, has an apparent MW of 125 kDa.

          靶标信息

          Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; p41/p43FRNK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; tyrosine kinase

          查看更多 收起

          基因别名: FADK; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2

          查看更多 收起

          UniProt ID: (Human) Q05397, (Mouse) P34152

          查看更多 收起

          Entrez Gene ID: (Human) 5747, (Chicken) 396416, (Mouse) 14083

          查看更多 收起

          功能
          actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding JUN kinase binding protein kinase binding SH2 domain binding protease binding calcium-dependent cysteine-type endopeptidase activity identical protein binding nucleotide binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding phosphatidylinositol 3-kinase binding
          参与通路
          MAPK cascade microtubule cytoskeleton organization angiogenesis blood vessel development vasculogenesis neuron migration regulation of protein phosphorylation positive regulation of protein phosphorylation protein phosphorylation movement of cell or subcellular component cell adhesion signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway JNK cascade multicellular organism development positive regulation of cell proliferation regulation of cell shape regulation of epithelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration phosphorylation peptidyl-tyrosine phosphorylation central nervous system neuron axonogenesis negative regulation of cell-cell adhesion regulation of cell adhesion extracellular matrix organization positive regulation of cell growth positive regulation of cell migration negative regulation of cell migration cellular chloride ion homeostasis regulation of cell adhesion mediated by integrin peptidyl-tyrosine autophosphorylation establishment of nucleus localization regulation of cell proliferation negative regulation of apoptotic process endothelial cell migration innate immune response regulation of osteoblast differentiation positive regulation of cell adhesion positive regulation of protein kinase activity positive regulation of vasodilation negative regulation of organ growth protein autophosphorylation ephrin receptor signaling pathway positive regulation of smooth muscle cell proliferation positive regulation of phagocytosis negative regulation of axonogenesis positive regulation of synaptic transmission regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of synapse assembly positive regulation of glial cell proliferation growth hormone receptor signaling pathway cellular response to transforming growth factor beta stimulus regulation of substrate adhesion-dependent cell spreading positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of anoikis placenta development heart morphogenesis cellular component disassembly involved in execution phase of apoptosis axon guidance embryo development regulation of endothelial cell migration establishment of cell polarity netrin-activated signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis regulation of GTPase activity positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity vascular endothelial growth factor receptor signaling pathway cell motility regulation of cytoskeleton organization proteolysis actin filament organization transmembrane receptor protein tyrosine kinase signaling pathway response to pH negative regulation of cell-substrate adhesion pyramidal neuron migration cell differentiation negative regulation of protein autophosphorylation positive regulation of protein binding response to muscle stretch wound healing, spreading of cells positive regulation of focal adhesion assembly angiogenesis involved in wound healing positive regulation of protein tyrosine kinase activity positive regulation of substrate-dependent cell migration, cell attachment to substrate
          It has to be done as per old AB suggested Products section.
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