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Invitrogen
Phospho-GCN2 (Thr899) Recombinant Rabbit Monoclonal Antibody (PSH10-57)
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Phospho-GCN2 (Thr899) Antibody (MA562894) in ICC/IF
Immunofluorescence analysis of Phospho-GCN2 (T899) using Neuro-2a cells untreated or treated with 100 nM Calyculin A for 30 minutes. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. The cells were co-labeled with Ph... View More
产品信息
MA562894
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH10-57
抗原
Synthetic phosphopeptide corresponding to residues surrounding Thr899 of Human GCN2.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Positive Control: HeLa cell lysate, HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate, Neuro-2a cell lysate, Neuro-2a treated with 100nM Calyculin A for 30 minutes cell lysate, C6 cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate, HeLa cells treated with 100nM Calyculin A for 30 minutes, Neuro-2a cells treated with 100nM Calyculin A for 30 minutes, C6 cells treated with 100ng/mL Calyculin A for 1 hour, human brain tissue, mouse brain tissue, rat brain tissue.
Tissue Specificity: Low tissue specificity.
Subcellular Location: Cytoplasm.
Sequence Similarities: 100% Mouse/Rat.
Predicted band size: 187 kDa.
靶标信息
EIF2AK4 (GCN2) is a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor-2 in response to low amino acid availability. It plays a role as an activator of the integrated stress response required for adapatation to amino acid starvation. Mutations in the gene lead to pulmonary vennocclusive disease 2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eIF-2-alpha kinase GCN2; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2 eIF2alpha kinase; GCN2-like protein; general control nonderepressible 2; mGCN2
基因别名: 2610011M03; EIF2AK4; GCN2; KIAA1338; MGCN2; PVOD2
UniProt ID: (Human) Q9P2K8, (Mouse) Q9QZ05
Entrez Gene ID: (Human) 440275, (Mouse) 27103, (Rat) 114859
tRNA binding
protein serine/threonine kinase activity
eukaryotic translation initiation factor 2alpha kinase activity
ATP binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
eukaryotic initiation factor eIF2 binding
aminoacyl-tRNA synthetase
non-receptor serine/threonine protein kinase
translational protein
positive regulation of defense response to virus by host
adaptive immune response
T cell activation involved in immune response
positive regulation of adaptive immune response
regulation of translational initiation
protein phosphorylation
cell cycle arrest
learning
long-term memory
regulation of translational initiation by eIF2 alpha phosphorylation
viral translation
negative regulation of translational initiation in response to stress
negative regulation of CREB transcription factor activity
cellular response to amino acid starvation
cellular response to UV
eiF2alpha phosphorylation in response to endoplasmic reticulum stress
induction by virus of host autophagy
negative regulation by host of viral genome replication
negative regulation of neuron differentiation
negative regulation of translational initiation
protein autophosphorylation
defense response to virus
regulation of feeding behavior
regulation of eIF2 alpha phosphorylation by amino acid starvation
cellular response to cold
positive regulation of translational initiation in response to starvation
positive regulation of long-term synaptic potentiation
neuron projection extension
cellular response to leucine starvation
cellular response to starvation
positive regulation of gene expression
phosphorylation
negative regulation of translation
endoplasmic reticulum unfolded protein response
response to endoplasmic reticulum stress
regulation of translational initiation in response to stress
