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          • Primary Antibodies ›
          • GSK3B Antibodies

          Bioss

          Phospho-GSK-3 Beta (Ser21, Ser29) Polyclonal Antibody

          查看其他47个GSK3B抗体

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          Cite Phospho-GSK-3 Beta (Ser21, Ser29) Polyclonal Antibody

          Additional Information:
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          • 抗体检测数据 (2)
          Phospho-GSK-3 Beta (Ser21, Ser29) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-GSK-3 Beta (Ser21, Ser29) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 2

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          Phospho-GSK-3 Beta (Ser21, Ser29) Antibody (BS-5367R) in ICC/IF

          A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-GSK-3 Beta (Ser21+Ser29)) polyclonal Antibody, Unconjugated (bs-5367R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
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          Phospho-GSK-3 Beta (Ser21, Ser29) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-GSK-3 Beta (Ser21, Ser29) Antibody in Western Blot (WB)
          Phospho-GSK-3 Beta (Ser21, Ser29) Polyclonal Antibody

          产品信息

          BS-5367R

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:300-1:5,000
          -

          免疫组化(石蜡) (IHC (P))

          1:200-400
          -

          免疫组化(冰冻) (IHC (F))

          1:100-500
          -

          免疫细胞化学 (ICC/IF)

          1:50-1:200
          -

          酶联免疫吸附实验 (ELISA)

          1:500-1:1,000
          -
          产品规格

          种属反应

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          KLH conjugated synthetic phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser21 & Ser29.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Protein A

          保存液

          TBS, pH 7.4, with 50% glycerol, 1% BSA

          内含物

          0.03% ProClin 300

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          靶标信息

          GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: EC 2.7.11.26; FA; Factor A; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK 3B; GSK-3 beta; GSK-3 beta GSK3B; GSK-3b; GSK3 beta; GSK3B; kinase; pp37; Serine/threonine-protein kinase GSK3B; unnamed protein product

          查看更多 收起

          基因别名: 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3; GSK3B

          查看更多 收起

          UniProt ID: (Human) P49841, (Mouse) Q9WV60, (Rat) P18266

          查看更多 收起

          Entrez Gene ID: (Human) 2932, (Mouse) 56637, (Rat) 84027

          查看更多 收起

          功能
          nucleotide binding protease binding p53 binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding beta-catenin binding kinase activity transferase activity protein kinase binding ubiquitin protein ligase binding protein kinase A catalytic subunit binding dynactin binding tau protein binding tau-protein kinase activity NF-kappaB binding RNA polymerase II sequence-specific DNA binding transcription factor binding scaffold protein binding protein serine kinase activity RNA polymerase II transcription factor binding integrin binding transcription factor binding transferase activity, transferring phosphorus-containing groups ionotropic glutamate receptor binding non-receptor serine/threonine protein kinase
          参与通路
          G1/S transition of mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion cytoplasmic translational initiation heart valve development glycogen metabolic process protein phosphorylation ER overload response mitochondrion organization regulation of mitotic cell cycle nervous system development circadian rhythm insulin receptor signaling pathway negative regulation of signal transduction positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression negative regulation of epithelial to mesenchymal transition regulation of neuron projection development Wnt signaling pathway peptidyl-serine phosphorylation viral protein processing hippocampus development establishment of cell polarity maintenance of cell polarity cell differentiation positive regulation of cell migration negative regulation of cell migration regulation of axon extension SCF-dependent proteasomal ubiquitin-dependent protein catabolic process neuron projection development negative regulation of protein complex assembly positive regulation of protein complex assembly positive regulation of protein ubiquitination negative regulation of TOR signaling positive regulation of protein binding negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of microtubule-based process response to endoplasmic reticulum stress intracellular signal transduction cellular response to interleukin-3 TORC2 signaling regulation of circadian rhythm negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process protein kinase B signaling positive regulation of neuron apoptotic process positive regulation of cell differentiation negative regulation of osteoblast differentiation negative regulation of glycogen biosynthetic process positive regulation of cilium assembly positive regulation of translation positive regulation of protein catabolic process positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation regulation of protein export from nucleus positive regulation of protein export from nucleus rhythmic process regulation of dendrite morphogenesis regulation of axonogenesis positive regulation of protein kinase B signaling canonical Wnt signaling pathway excitatory postsynaptic potential regulation of microtubule cytoskeleton organization negative regulation of calcineurin-NFAT signaling cascade superior temporal gyrus development cellular response to retinoic acid negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand presynaptic modulation of chemical synaptic transmission neuron projection organization membraneless organelle assembly regulation of microtubule anchoring at centrosome beta-arrestin-dependent dopamine receptor signaling pathway regulation of cellular response to heat negative regulation of protein localization to nucleus regulation of long-term synaptic potentiation positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway cellular response to oxygen-containing compound negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of protein localization to cilium negative regulation of TORC2 signaling negative regulation of dopaminergic neuron differentiation cellular response to beta-amyloid positive regulation of protein localization to centrosome positive regulation of establishment of protein localization negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of type B pancreatic cell development negative regulation of glycogen (starch) synthase activity negative regulation of mesenchymal stem cell differentiation re-entry into mitotic cell cycle carbohydrate metabolic process regulation of gene expression by genetic imprinting protein export from nucleus cytoskeleton organization establishment or maintenance of cell polarity multicellular organism development axonogenesis myoblast fusion cell proliferation organ morphogenesis negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation phosphorylation cell migration negative regulation of protein binding positive regulation of peptidyl-serine phosphorylation protein localization to microtubule positive regulation of apoptotic process negative regulation of MAP kinase activity positive regulation of GTPase activity hypermethylation of CpG island canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation positive regulation of axon extension regulation of neuronal synaptic plasticity negative regulation of dendrite morphogenesis negative regulation of NFAT protein import into nucleus cellular response to mechanical stimulus positive regulation of stem cell differentiation response to lithium ion response to drug
          It has to be done as per old AB suggested Products section.

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