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          • Primary Antibodies ›
          • GSK3 alpha/beta Antibodies

          Abnova

          Phospho-GSK3A/GSK3B (Tyr216, Tyr279) Recombinant Rabbit Monoclonal Antibody

          查看其他19个GSK3 alpha/beta抗体

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          Cite Phospho-GSK3A/GSK3B (Tyr216, Tyr279) Recombinant Rabbit Monoclonal Antibody

          Additional Information:

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          • 抗体检测数据 (1)
          Phospho-GSK3A/GSK3B (Tyr216, Tyr279) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-GSK3A/GSK3B (Tyr216, Tyr279) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 1

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          Phospho-GSK3A/GSK3B (Tyr216, Tyr279) Antibody (RAB02237) in WB

          Western Blot analysis of Lane 1: C6 and Lane 2: 3T3 lysates with GSK3A/GSK3B (phospho Tyr216/Tyr279) recombinant monoclonal antibody, clone R01-5G6 (Product # RAB02237). {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          Phospho-GSK3A/GSK3B (Tyr216, Tyr279) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-GSK3A/GSK3B (Tyr216, Tyr279) Recombinant Rabbit Monoclonal Antibody

          产品信息

          RAB02237

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:500-1:1,000
          -

          免疫沉淀 (IP)

          1:20
          -
          产品规格

          种属反应

          Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          Expression System

          CHO cells

          分类

          Recombinant Monoclonal

          类型

          Antibody

          抗原

          Synthetic phosphopeptide corresponding to residues surrounding Tyr216 of human GSK3A/GSK3B.
          3D表位/免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          纯化类型

          Affinity chromatography

          保存液

          50mM tris glycine, pH 7.4, with 150mM NaCl, 0.05% BSA, 40% glycerol

          内含物

          0.01% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          靶标信息

          Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          Cite this product

          生物信息学

          蛋白别名: FA; factor A; GSK-3 alpha; GSK-3 beta; gsk-3-alpha; mGSK3beta; serine/threonine-protein kinase GSK3A; serine/threonine-protein kinase GSK3B; unnamed protein product

          查看更多 收起

          基因别名: 2700086H06Rik; 7330414F15Rik; 8430431H08Rik; GSK-3; GSK-3beta; GSK3

          查看更多 收起

          UniProt ID: (Rat) P18265, (Mouse) Q2NL51, (Mouse) Q9WV60, (Rat) P18266

          查看更多 收起

          Entrez Gene ID: (Rat) 50686, (Mouse) 606496, (Mouse) 56637, (Rat) 84027

          查看更多 收起

          功能
          protein kinase activity protein serine/threonine kinase activity receptor binding ATP binding protein kinase binding protein kinase A catalytic subunit binding tau-protein kinase activity protein serine kinase activity protein serine/threonine kinase binding protein binding protease binding p53 binding integrin binding beta-catenin binding kinase activity ubiquitin protein ligase binding dynactin binding ionotropic glutamate receptor binding tau protein binding NF-kappaB binding RNA polymerase II sequence-specific DNA binding transcription factor binding dynein complex binding scaffold protein binding
          参与通路
          regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis protein phosphorylation insulin receptor signaling pathway positive regulation of autophagy positive regulation of gene expression positive regulation of peptidyl-threonine phosphorylation regulation of neuron projection development cell migration cell differentiation positive regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway negative regulation of TOR signaling positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation cellular response to interleukin-3 proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of neuron apoptotic process positive regulation of heart contraction positive regulation of transcription from RNA polymerase II promoter negative regulation of glucose import negative regulation of insulin receptor signaling pathway negative regulation of cell growth involved in cardiac muscle cell development regulation of microtubule cytoskeleton organization cellular response to lithium ion cellular response to glucocorticoid stimulus positive regulation of adrenergic receptor signaling pathway negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway autosome genomic imprinting positive regulation of substrate adhesion-dependent cell spreading positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway regulation of mitophagy positive regulation of beta-amyloid formation positive regulation of establishment of protein localization to mitochondrion negative regulation of dendrite development regulation of cell growth negative regulation of cardiac muscle hypertrophy negative regulation of developmental process regulation of striated muscle cell differentiation G1/S transition of mitotic cell cycle re-entry into mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion cytoplasmic translational initiation glycogen metabolic process protein export from nucleus ER overload response mitochondrion organization cytoskeleton organization meiosis I establishment or maintenance of cell polarity regulation of mitotic cell cycle axonogenesis myoblast fusion circadian rhythm organ morphogenesis response to zinc ion negative regulation of gene expression negative regulation of epithelial to mesenchymal transition positive regulation of mitochondrial membrane potential positive regulation of protein processing negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation Wnt signaling pathway phosphorylation peptidyl-serine phosphorylation hippocampus development establishment of cell polarity maintenance of cell polarity positive regulation of cell migration negative regulation of cell migration regulation of axon extension SCF-dependent proteasomal ubiquitin-dependent protein catabolic process neuron projection development negative regulation of protein complex assembly positive regulation of protein complex assembly negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of type I interferon production regulation of protein localization regulation of microtubule-based process positive regulation of osteoblast proliferation negative regulation of smooth muscle cell apoptotic process response to endoplasmic reticulum stress protein localization to microtubule intracellular signal transduction cellular response to hepatocyte growth factor stimulus hepatic stellate cell activation TORC2 signaling regulation of circadian rhythm regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of MAP kinase activity protein kinase B signaling fat cell differentiation positive regulation of cell differentiation regulation of osteoblast differentiation negative regulation of osteoblast differentiation positive regulation of osteoclast differentiation negative regulation of glycogen biosynthetic process positive regulation of cilium assembly positive regulation of translation positive regulation of protein catabolic process positive regulation of axon extension negative regulation of smoothened signaling pathway negative regulation of synaptic growth at neuromuscular junction positive regulation of synaptic growth at neuromuscular junction protein autophosphorylation regulation of protein export from nucleus positive regulation of protein export from nucleus bone remodeling regulation of neuronal synaptic plasticity positive regulation of smooth muscle cell proliferation regulation of dendrite morphogenesis regulation of axonogenesis negative regulation of dendrite morphogenesis negative regulation of nitric-oxide synthase activity positive regulation of protein kinase B signaling canonical Wnt signaling pathway cell growth involved in cardiac muscle cell development intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress negative regulation of calcineurin-NFAT signaling cascade superior temporal gyrus development cellular response to mechanical stimulus cellular response to retinoic acid positive regulation of osteoclast proliferation hepatocyte apoptotic process regulation of neurotransmitter receptor localization to postsynaptic specialization membrane regulation of modification of postsynaptic structure presynaptic modulation of chemical synaptic transmission neuron projection organization regulation of microtubule anchoring at centrosome negative regulation of protein localization to nucleus regulation of long-term synaptic potentiation negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of protein localization to cilium negative regulation of TORC2 signaling response to methamphetamine hydrochloride negative regulation of dopaminergic neuron differentiation cellular response to beta-amyloid negative regulation of protein localization to centrosome positive regulation of protein localization to centrosome conditioned place preference negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of excitatory postsynaptic potential positive regulation of DNA biosynthetic process positive regulation of cardiac muscle cell differentiation positive regulation of stem cell differentiation negative regulation of mesenchymal stem cell differentiation negative regulation of neuron migration response to xenobiotic stimulus response to activity positive regulation of protein binding response to estradiol response to insulin cellular response to erythropoietin cellular response to hydrogen peroxide cellular response to iron(II) ion response to epinephrine response to L-glutamate cellular response to dopamine response to insulin-like growth factor stimulus response to ultrasound response to angiotensin
          It has to be done as per old AB suggested Products section.

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