Invitrogen

Phospho-Histone H3 (Ser10) Polyclonal Antibody

高级验证

This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.

15个已发表图片

18篇引用文献

查看其他112个Histone H3抗体

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Phospho-Histone H3 (Ser10) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Phospho-Histone H3 (ser 10) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Histone H3 (Ser10) Rabbit Polyclonal antibody (Product # PA5-17869) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization in dividing cells. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Phospho-Histone H3 (Ser10) Antibody in Immunohistochemistry (IHC) — Immunohistochemistry was performed on paraffin-embedded human breast carcinoma. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes at 95C. Following antigen-retrieval, tissues were incubated in 3% hydrogen peroxide for 10 minutes, washed, and then blocked in 5% normal goat serum in TBST for 1 hour at room temperature. Tissues were stained with a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:200 overnight at 4C. Tissues were washed extensively with TBS + 0.1% Tween-20 and detection was performed using a HRP-conjugated secondary reagent followed by DAB substrate.

Phospho-Histone H3 (Ser10) Antibody in Western Blot (WB) — Western blot analysis was performed on acid extracts (20 µg lysate) of HL-60 (Lane 1), HL-60 treated with Calyculin A (50 ng/mL for 30 minutes) (Lane 2), NIH/3T3 (Lane 3) and NIH/3T3 treated with Nocodazole (100 ng/mL for 18 hours) (Lane 4). The blot was probed with Anti-Phospho-Histone H3 (Ser10) (Product # PA5-17869, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Phospho-Histone H3 (Ser10) was observed across the cell lines tested and enhanced upon treatment.

Phospho-Histone H3 (Ser10) Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Phospho-Histone H3 pSer10 on Ramos cells left untreated (blue histogram) or treated with serµm/calyculin (green histogram). Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Histone H3 pSer10 polyclonal antibody (Product # PA5-17869) at a dilution of 1:50 for 1 hour at room temperature. As a negative control, serµm/calyculin-treated cells were stained with an isotype control (red histogram). Following staining with either PA5-17869 or isotype control, cells were stained with a fluorescently-conjugated anti-rabbit IgG secondary antibody and analyzed on a flow cytometer.

Phospho-Histone H3 (Ser10) Antibody in Peptide array (Array) — The specificity of the antibody, Anti-Phospho-Histone H3 (Ser10) Polyclonal Antibody (Product # PA5-17869, 1:2000 dilution) to Histone H3pS10 peptide was confirmed using MODified™ Histone Peptide Array (Active Motif, 13001). Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) was used as the secondary antibody. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). The dot blot data obtained (lower panel) was analyzed using Array Analyze software as per the manufacturer's instructions (top panel).

Phospho-Histone H3 (Ser10) Antibody — Detection of differential subcellular localization of the target protein demonstrates antibody specificity. Immunofluorescence analysis of Phospho-Histone H3(Ser10) using anti-Phospho-Histone H3(Ser10) Polyclonal antibody (Product # PA5-17869 ) shows localization of Phospho-Histone H3 (Ser 10) predominantly in nucleus of dividing HeLa cells. {RE}

Phospho-Histone H3 (Ser10) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — 5 Figure Progesterone promotes CM proliferation through up-regulation of YAP expression. Cultured P7 CMs were treated with control (vehicle, DMSO) or progesterone (10 -7 M), alone or in combination with RU486 (A-C) or YAP siRNAs (D-F). A, Proliferation regulator mRNA expressions of CM were analysed by quantitative PCR (n = 4). B, Representative immunoblots (B1) and quantification (B2) of YAP protein expression were analysed by Western blotting (GAPDH was used as internal control) (n = 4). C, The mRNA expression of YAP target genes ANKRD1, CYR61 and CTGF was analysed by quantitative real-time PCR (n = 4). D-F, CM proliferation was evaluated by Ki67, PH3 and Aurora B immunostaining. Representative images are shown in D1, E1 and F1, and quantification of percentages of Ki67 + , PH3 + or Aurora B + CMs is shown in D2, E2 and F2, respectively (3000-5000 cells were randomly selected and analysed for each group) (n = 4). Scale bars are 40 um ( ** P < .01, *** P < .001) vs Control; ( ## P < .01, ### P < .001) vs Progesterone. CM, cardiomyocyte; YAP, yes-associated protein

Phospho-Histone H3 (Ser10) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Figure 1. CAF1 cells are mitotically active. (A) Identification of human primary ovarian NFs and CAFs. Immunostaining of FSP1 and western blot analysis of alpha-SMA in NFs and CAFs. Cells were stained with FSP1 (green) and counterstained with DAPI (blue). The relative intensity of alpha-SMA normalized to housekeeping protein was shown at the bottom. (B) Cell cycle analysis of NFs and CAFs. (C) The cell cycle phase distribution in each cell line (as in B). (D) Pathways enriched in CAF1 was revealed by GSEA indicated that CAF1 cells were mitotically active. (E) Immunostaining of phospho-histone H3 in NF1 and CAF1 cells. Cells were stained with phospho-histone H3 (green) and counterstained with DAPI (blue). (F) Western blot analysis of phospho-histone H3 in NF1 and CAF1 cells. The relative intensity of phospho-histone H3 normalized to housekeeping protein was shown at the bottom.

Phospho-Histone H3 (Ser10) Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — 2 Figure Progesterone supplementation promotes postnatal CM proliferation in vivo. A, Overview of the experimental set-up of the daily intraperitoneal injection of progesterone (8 mg/kg) or control vehicle (corn oil) from P1 to P6. Hearts were harvested at P7. B-D, CM proliferation was evaluated by Ki67, PH3 and Aurora B immunostaining. Representative images with z-stacking are shown in B1, C1 and D1, and quantification of percentages of Ki67 + , PH3 + or Aurora B + CMs is shown in B2, C2 and D2, respectively (n = 6). Scale bars are 20 um. E, Representative images (E1) and quantification (E2) of the cell size of CMs (labelled by cTnT and pan-cadherin to show intact cardiomyocytes) isolated from P7 heart. Eighty to one hundred cells were randomly selected per heart (n = 6). Scale bars are 20 um. F, Heart weight and body weight (HW/BW) ratio (n = 10-11). G, The mRNA expression of cell cycle activators in mouse hearts was analysed by real-time PCR (n = 5). CM, cardiomyocyte

产品信息

PA5-17869

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:1,000	查看7篇已发表文章 7篇已发表文章
免疫组化 (IHC)	-	查看6篇已发表文章 6篇已发表文章
免疫组化（石蜡） (IHC (P))	1:200	-
免疫组织化学（多聚甲醛固定） (IHC (PFA))	-	查看1篇已发表文章 1篇已发表文章
免疫组化（冰冻） (IHC (F))	1:400	-
免疫细胞化学 (ICC/IF)	1:800	查看4篇已发表文章 4篇已发表文章
流式细胞分析 (Flow)	1:50	-
肽阵列 (Array)	1:2,000	-

产品规格
种属反应	Fruit fly, Human, Mouse, Non-human primate, Rat, Xenopus
已发表种属	Fruit fly, Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic phosphopeptide corresponding to residues surrounding pSer10 of human histone H3
偶联物	Unconjugated
形式	Liquid
纯化类型	Antigen affinity chromatography
保存液	0.01M HEPES, pH 7.5, with 100µg/mL BSA, 0.15M NaCl, 50% glycerol
内含物	no preservative
保存条件	-20°C
RRID	AB_10984484

产品详细信息

Phospho-Histone H3 (Ser10) Antibody detects endogenous levels of histone H3 only when phosphorylated at Ser10; however, this antibody does not detect phosphorylated Ser10 when Lys9 is acetylated or methylated. This antibody does not cross-react with histone H3 phosphorylated at Ser28.

This antibody is not cross-reactive with other phosphorylated histones or with acetylated histones.

靶标信息

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CG31613-PA; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3S10ph; His3:CG31613-PA; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; Histone Cluster 2 H3a; histone cluster 2, H3c2, pseudogene; histone gene complex 1; Histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o

基因别名: CG31613; CG33803; CG33806; CG33809; CG33812; CG33815; CG33818; CG33821; CG33824; CG33827; CG33830; CG33833; CG33836; CG33839; CG33842; CG33845; CG33848; CG33851; CG33854; CG33857; CG33860; CG33863; CG33866; Dmel\CG31613; Dmel_CG31613; H3; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; h3.2a; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; h3r; His3; His3:CG31613; His3:CG33803; His3:CG33806; His3:CG33809; His3:CG33812; His3:CG33815; His3:CG33818; His3:CG33821; His3:CG33824; His3:CG33827; His3:CG33830; His3:CG33833; His3:CG33836; His3:CG33839; His3:CG33842; His3:CG33845; His3:CG33848; His3:CG33851; His3:CG33854; His3:CG33857; His3:CG33860; His3:CG33863; His3:CG33866; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; hist2h3; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461; XELAEV_18028537mg

UniProt ID: (Human) P68431, (Human) Q71DI3, (Fruit fly) P02299, (Mouse) P68433, (Mouse) P84228, (Mouse) P17320

Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Fruit fly) 318847, (Mouse) 360198, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114, (Xenopus) 399088

分子生物学功能: histone

功能

chromatin silencing at rDNA positive regulation of defense response to virus by host nucleosome assembly DNA replication-dependent nucleosome assembly blood coagulation gene silencing by RNA telomere organization cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic protein heterotetramerization regulation of gene silencing cell-cell adhesion xenophagy

参与通路

DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion nucleosomal DNA binding

Phospho-Histone H3 (Ser10) Polyclonal Antibody

产品信息

PA5-17869

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组织化学（多聚甲醛固定） (IHC (PFA))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

肽阵列 (Array)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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