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Invitrogen
Phospho-IGF1R beta (Tyr1135, Tyr1136) Recombinant Rabbit Monoclonal Antibody (15H10L8)
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Phospho-IGF1R beta (Tyr1135, Tyr1136) Antibody (701067) in WB
Western blot analysis of Phospho-IGF1R pTyr1135/1136 in whole cell extracts of HeLa cells treated with Insulin (100 ng/mL, 15 min) using a Phospho-IGF1R pTyr1135/1136 recombinant rabbit monoclonal antibody (Product # 701067) at a dilution of 2.5 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lane 1). Results show a band at ~90 k... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
701067
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
15H10L8
抗原
Phosphopeptide corresponding to amino acids 1131-1140 of human IGF1R
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: IGF-I receptor
基因别名: CD221; IGFIR; IGFR; JTK13
UniProt ID: (Human) P08069
Entrez Gene ID: (Human) 3480
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
insulin-activated receptor activity
insulin-like growth factor-activated receptor activity
insulin receptor binding
structural molecule activity
protein binding
insulin-like growth factor binding
ATP binding
insulin-like growth factor I binding
identical protein binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
immune response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of cell migration
peptidyl-tyrosine autophosphorylation
negative regulation of apoptotic process
positive regulation of protein complex disassembly
negative regulation of MAPK cascade
positive regulation of MAPK cascade
protein kinase B signaling
transcytosis
regulation of JNK cascade
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
positive regulation of protein kinase B signaling
cellular response to glucose stimulus
dendritic spine maintenance
beta-amyloid clearance
positive regulation of cold-induced thermogenesis
cellular response to oxygen-containing compound
cellular response to beta-amyloid
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
