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Invitrogen
Phospho-IRS1 (Ser312) Recombinant Superclonal Antibody (18HCLC)
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
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Phospho-IRS1 (Ser312) Antibody (710778) in ICC/IF
Immunofluorescence was performed on MCF7 cells treated with IGF (100 ng/mL/20 min). These cells were permeabilized and fixed for detection of IRS (pS312) using Anti-IRS (pS312) Recombinant Rabbit Polyclonal Antibody (Product # 710778, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows r... View More
产品信息
710778
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
18HCLC
抗原
Phosphopeptide corresponding to Human IRS1 Ser312 (aa 309-318)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532762
产品详细信息
This antibody is predicted to react with Monkey, Bovine, Horse and Cat.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Insulin receptor substrate 1; IRS-1
基因别名: G972R; HIRS-1; IRS-1; IRS1
UniProt ID: (Human) P35568, (Mouse) P35569
Entrez Gene ID: (Human) 3667, (Mouse) 16367
signal transducer activity
transmembrane receptor protein tyrosine kinase adaptor activity
protein kinase C binding
Ras guanyl-nucleotide exchange factor activity
insulin receptor binding
insulin-like growth factor receptor binding
protein binding
1-phosphatidylinositol-3-kinase activity
SH2 domain binding
phosphatidylinositol 3-kinase binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
protein kinase binding
protein domain specific binding
MAPK cascade
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of glucose metabolic process
phosphatidylinositol 3-kinase signaling
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of fatty acid beta-oxidation
response to insulin
cellular response to insulin stimulus
phosphatidylinositol-3-phosphate biosynthetic process
glucose homeostasis
response to peptide hormone
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of glycogen biosynthetic process
positive regulation of glucose import
negative regulation of insulin receptor signaling pathway
positive regulation of insulin receptor signaling pathway
negative regulation of insulin secretion
phosphatidylinositol phosphorylation
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of glucose import in response to insulin stimulus
positive regulation of mesenchymal cell proliferation
regulation of gene expression
lipid catabolic process
insulin secretion
positive regulation of cell migration
mammary gland development
response to caffeine
protein localization to nucleus
positive regulation of phosphorylation
protein kinase B signaling
protein heterooligomerization
positive regulation of glucagon secretion
negative regulation of somatostatin secretion
