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Phospho-JAK1 (Tyr1034, Tyr1035) Antibody (700028) in ICC/IF
Immunofluorescence analysis of JAK1 (pY1034/1035) was done on 70% confluent log phase COLO 205 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with JAK1 (pY1034/1035) Recombinant Rabbit Monoclonal Antibody (Product # 700028) at 2 µg/mL in 1% BSA and incuba... View More
产品信息
700028
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
59H4L5
抗原
A peptide corresponding to amino acids 1030-1039 of P23458. This site is commonly referred to as pY1022/pY1023 in the literature.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with mouse, rat, chimpanzee, Rhesus monkey, bovine, canine, porcine, equine, Xenopus and zebrafish based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This product was formerly called "Phospho-JAK1 (Tyr1022, Tyr1023)" because Human Jak1 residues Tyr1034 and Tyr1035 have been historically referenced as Tyr1022 and Tyr1023.
靶标信息
Janus kinase 1 (JAK1), is a protein tyrosine kinase that is involved in the interferon-alpha/beta and -gamma signal transduction pathways. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK1. JAK1 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. Upon ligand binding, JAK1 undergoes tyrosine phosphorylation and catalytic activation in an interdependent manner. Phosphorylation of tyrosine residues at positions 1022 and 1023 is believed to function in the activation of catalytic events.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: EC 2.7.10.2; JAK-1; Janus kinase 1; kinase Jak1; Tyrosine-protein kinase JAK1
基因别名: AA960307; BAP004; C130039L05Rik; JAK1; JAK1A; JAK1B; JTK3
UniProt ID: (Human) P23458
Entrez Gene ID: (Human) 3716, (Mouse) 16451
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
receptor binding
growth hormone receptor binding
protein binding
ATP binding
protein phosphatase binding
ubiquitin protein ligase binding
CCR5 chemokine receptor binding
metal ion binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
MAPK cascade
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
cell migration
cell differentiation
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
interleukin-2-mediated signaling pathway
regulation of cell proliferation
positive regulation of GTPase activity
innate immune response
response to antibiotic
interferon-gamma-mediated signaling pathway
regulation of interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
regulation of type I interferon-mediated signaling pathway
positive regulation of sprouting angiogenesis
enzyme linked receptor protein signaling pathway
phosphorylation
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
protein autophosphorylation
