Invitrogen

Phospho-PAK1/2/3 (Ser141) Polyclonal Antibody

Phospho-PAK1/2/3 (Ser141) Antibody in Western Blot (WB)

图: 1 / 20

Phospho-PAK1/2/3 (Ser141) Antibody (44-940G) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y treated with 100 uM Pervanadate for 1 hour (Lane 2), HeLa (Lane 3), HeLa treated with 100 uM Pervanadate for 1 hour (Lane 4), K562 (Lane 5), K562 treated with 100 uM Pervanadate for 1 hour (Lane 6). The blots were probed with Anti-Phospho-PAK1 + 2 + 3 pSer141 Rabbit Polyclonal Antibody (Product # 44-940G, ... View More

Phospho-PAK1/2/3 (Ser141) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Phospho-PAK1/2/3 (Ser141) Antibody in Immunocytochemistry (ICC/IF)

Phospho-PAK1/2/3 (Ser141) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

44-940G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看15篇已发表文章 15篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看3篇已发表文章 3篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Bovine, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phosphopeptide derived from a region of human PAK2 containing serine 141. The sequence is conserved in rat and rabbit.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533801

靶标信息

In mammals, there are several identified isoforms of p21-Activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated wit X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-β-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learning.

Molecular psychiatry

Yamahashi Y,Lin YH,Mouri A,Iwanaga S,Kawashima K,Tokumoto Y,Watanabe Y,Faruk MO,Zhang X,Tsuboi D,Nakano T,Saito N,Nagai T,Yamada K,Kaibuchi K

44-940G was used in Immunohistochemistry to demonstrate that acetylcholine stimulates M1R-PKC-β-PIX-Rac1-PAK signaling in D2R-MSNs for aversive learning and imply the cascade's therapeutic potential for AD as aversive learning is used to preliminarily screen AD drugs.

Mon Aug 01 00:00:00 UTC 2022

Activating RAC1 variants in the switch II region cause a developmental syndrome and alter neuronal morphology.

Brain : a journal of neurology

Banka S,Bennington A,Baker MJ,Rijckmans E,Clemente GD,Ansor NM,Sito H,Prasad P,Anyane-Yeboa K,Badalato L,Dimitrov B,Fitzpatrick D,Hurst ACE,Jansen AC,Kelly MA,Krantz I,Rieubland C,Ross M,Rudy NL,Sanz J,Stouffs K,Xu ZL,Malliri A,Kazanietz MG,Millard TH

44-940G was used in Western Blot, Immunocytochemistry to reveal that these variants cause altered downstream signalling, resulting in abnormal neuronal morphology and reveal the WAVE regulatory complex/Arp2/3 pathway as a possible therapeutic target for activating RAC1 variants.

Mon Dec 19 00:00:00 UTC 2022

Nck1, But Not Nck2, Mediates Disturbed Flow-Induced p21-Activated Kinase Activation and Endothelial Permeability.

Journal of the American Heart Association

Alfaidi M,Bhattarai U,Orr AW

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Western Blot

Tue Jun 02 00:00:00 UTC 2020

Nck1, But Not Nck2, Mediates Disturbed Flow-Induced p21-Activated Kinase Activation and Endothelial Permeability.

Journal of the American Heart Association

Alfaidi M,Bhattarai U,Orr AW

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Western Blot

Tue Jun 02 00:00:00 UTC 2020

Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Yang CY,Yu TH,Wen WL,Ling P,Hsu KS

44-940G was used in Western Blotting to establish a critical link between elevated Rac1 activity, structural and synaptic plasticity alterations, and cognitive impairment caused by Cc2d1a deletion.

Wed Jun 19 00:00:00 UTC 2019

Group I Paks are essential for epithelial- mesenchymal transition in an Apc-driven model of colorectal cancer.

Nature communications

Chow HY,Dong B,Valencia CA,Zeng CT,Koch JN,Prudnikova TY,Chernoff J

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Western Blot

Mon Aug 27 00:00:00 UTC 2018

Transgenic autoinhibition of p21-activated kinase exacerbates synaptic impairments and fronto-dependent behavioral deficits in an animal model of Alzheimer's disease.

Aging

Bories C,Arsenault D,Lemire M,Tremblay C,De Koninck Y,Calon F

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Western Blot

Tue May 16 00:00:00 UTC 2017

The calcium sensor Copine-6 regulates spine structural plasticity and learning and memory.

Nature communications

Reinhard JR,Kriz A,Galic M,Angliker N,Rajalu M,Vogt KE,Ruegg MA

44-940G was used in immunocytochemistry to determine regulation of spine structural plasticity and memory and learning by the calcium sensor Copine-6

Thu May 19 00:00:00 UTC 2016

A bead-based western for high-throughput cellular signal transduction analyses.

Nature communications

Treindl F,Ruprecht B,Beiter Y,Schultz S,Döttinger A,Staebler A,Joos TO,Kling S,Poetz O,Fehm T,Neubauer H,Kuster B,Templin MF

44-940G was used in western blot identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance

Fri Sep 23 00:00:00 UTC 2016

The matrikine N-α-PGP couples extracellular matrix fragmentation to endothelial permeability.

Science advances

Hahn CS,Scott DW,Xu X,Roda MA,Payne GA,Wells JM,Viera L,Winstead CJ,Bratcher P,Sparidans RW,Redegeld FA,Jackson PL,Folkerts G,Blalock JE,Patel RP,Gaggar A

44-940G was used in western blot to assess the contribution of tissue damage and extracellular matrix fragmentation during inflammation.

Mon Oct 19 00:00:00 UTC 2020

Group I Paks as therapeutic targets in NF2-deficient meningioma.

Oncotarget

Chow HY,Dong B,Duron SG,Campbell DA,Ong CC,Hoeflich KP,Chang LS,Welling DB,Yang ZJ,Chernoff J

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Western Blot

Tue Feb 10 00:00:00 UTC 2015

Pharmacological stimulation of the brain serotonin receptor 7 as a novel therapeutic approach for Rett syndrome.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology

De Filippis B,Nativio P,Fabbri A,Ricceri L,Adriani W,Lacivita E,Leopoldo M,Passarelli F,Fuso A,Laviola G

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Western Blot

Wed Oct 01 00:00:00 UTC 2014

Lis1 mediates planar polarity of auditory hair cells through regulation of microtubule organization.

Development (Cambridge, England)

Sipe CW,Liu L,Lee J,Grimsley-Myers C,Lu X

44-940G was used in western blot to elucidate the hair cell-intrinsic polarity machinery.

Mon Apr 01 00:00:00 UTC 2013

Glucocorticoid receptors are localized to dendritic spines and influence local actin signaling.

Molecular neurobiology

Jafari M,Seese RR,Babayan AH,Gall CM,Lauterborn JC

44-940G was used in western blot to study glucocorticoid signaling in the spine.

Mon Oct 01 00:00:00 UTC 2012

Glucocorticoid receptors are localized to dendritic spines and influence local actin signaling.

Molecular neurobiology

Jafari M,Seese RR,Babayan AH,Gall CM,Lauterborn JC

44-940G was used in western blot to study glucocorticoid signaling in the spine.

Mon Oct 01 00:00:00 UTC 2012

Physiological activation of synaptic Rac>PAK (p-21 activated kinase) signaling is defective in a mouse model of fragile X syndrome.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Chen LY,Rex CS,Babayan AH,Kramár EA,Lynch G,Gall CM,Lauterborn JC

Wed Aug 18 00:00:00 UTC 2010

Exercise improves cognition and hippocampal plasticity in APOE epsilon4 mice.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Nichol K,Deeny SP,Seif J,Camaclang K,Cotman CW

Wed Jul 01 00:00:00 UTC 2009

Loss of modifier of cell adhesion reveals a pathway leading to axonal degeneration.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Chen Q,Peto CA,Shelton GD,Mizisin A,Sawchenko PE,Schubert D

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Western Blot

Wed Jan 07 00:00:00 UTC 2009

Cytoskeletal changes underlie estrogen's acute effects on synaptic transmission and plasticity.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kramár EA,Chen LY,Brandon NJ,Rex CS,Liu F,Gall CM,Lynch G

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Western Blot

Wed Oct 14 00:00:00 UTC 2009

Signal transduction in Alzheimer disease: p21-activated kinase signaling requires C-terminal cleavage of APP at Asp664.

Journal of neurochemistry

Nguyen TV,Galvan V,Huang W,Banwait S,Tang H,Zhang J,Bredesen DE

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Immunohistochemistry

Fri Feb 01 00:00:00 UTC 2008

Signal transduction in Alzheimer disease: p21-activated kinase signaling requires C-terminal cleavage of APP at Asp664.

Journal of neurochemistry

Nguyen TV,Galvan V,Huang W,Banwait S,Tang H,Zhang J,Bredesen DE

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Immunohistochemistry

Fri Feb 01 00:00:00 UTC 2008

Signal transduction in Alzheimer disease: p21-activated kinase signaling requires C-terminal cleavage of APP at Asp664.

Journal of neurochemistry

Nguyen TV,Galvan V,Huang W,Banwait S,Tang H,Zhang J,Bredesen DE

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Immunohistochemistry

Fri Feb 01 00:00:00 UTC 2008

Signal transduction in Alzheimer disease: p21-activated kinase signaling requires C-terminal cleavage of APP at Asp664.

Journal of neurochemistry

Nguyen TV,Galvan V,Huang W,Banwait S,Tang H,Zhang J,Bredesen DE

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Immunohistochemistry

Fri Feb 01 00:00:00 UTC 2008

Signal transduction in Alzheimer disease: p21-activated kinase signaling requires C-terminal cleavage of APP at Asp664.

Journal of neurochemistry

Nguyen TV,Galvan V,Huang W,Banwait S,Tang H,Zhang J,Bredesen DE

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Immunohistochemistry

Fri Feb 01 00:00:00 UTC 2008

Signal transduction in Alzheimer disease: p21-activated kinase signaling requires C-terminal cleavage of APP at Asp664.

Journal of neurochemistry

Nguyen TV,Galvan V,Huang W,Banwait S,Tang H,Zhang J,Bredesen DE

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Immunohistochemistry

Fri Feb 01 00:00:00 UTC 2008

Brain-derived neurotrophic factor promotes long-term potentiation-related cytoskeletal changes in adult hippocampus.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Rex CS,Lin CY,Kramár EA,Chen LY,Gall CM,Lynch G

Published figure using Phospho-PAK1/2/3 (Ser141) polyclonal antibody (Product # 44-940G) in Western Blot

Wed Mar 14 00:00:00 UTC 2007

Changes in synaptic morphology accompany actin signaling during LTP.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Chen LY,Rex CS,Casale MS,Gall CM,Lynch G

Wed May 16 00:00:00 UTC 2007

p21-activated kinase regulates endothelial permeability through modulation of contractility.

The Journal of biological chemistry

Stockton RA,Schaefer E,Schwartz MA

Fri Nov 05 00:00:00 UTC 2004

p21-activated kinase regulates endothelial permeability through modulation of contractility.

The Journal of biological chemistry

Stockton RA,Schaefer E,Schwartz MA

Fri Nov 05 00:00:00 UTC 2004

生物信息学

蛋白别名: ADRB2; adriamycin resistance-associated; Alpha-PAK; Beta-PAK; CDKN1A; EC 2.7.11.1; gamma pak; Gamma-PAK; hPAK3; kinase PAK1; Oligophrenin-3; p21 (CDKN1A)-activated kinase 2; p21 (CDKN1A)-activated kinase 3; p21 protein (Cdc42/Rac)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 3; p21-activated kinase 1; p21-activated kinase 2; p21-activated kinase 3; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p58; p65-PAK; PAK 1; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK-3; PAK65; S6/H4 kinase; Serine/threonine-protein kinase PAK 1; Serine/threonine-protein kinase PAK 2; Serine/threonine-protein kinase PAK 3; STE20 homolog, yeast

查看更多收起

基因别名: ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK1; PAK2; PAK3; PAK3beta; PAK65; PAKalpha; PAKgamma

查看更多收起

UniProt ID: (Human) Q13153, (Human) Q13177, (Human) O75914

查看更多收起

Entrez Gene ID: (Human) 5058, (Human) 5062, (Human) 5063

查看更多收起

功能