Invitrogen

Phospho-PAK4/6/7 (Ser474) Polyclonal Antibody

产品信息

PA5-64495

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:1,000	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human, Mouse, Rat
已发表种属	Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	A synthesized peptide derived from human PAK4/5/6 (Phospho-Ser474) 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.4, with 50% glycerol
内含物	0.02% sodium azide
保存条件	-20°C
RRID	AB_2663447

产品详细信息

Phospho-PAK4/PAK5/PAK6 (Ser474) Polyclonal Antibody detects endogenous levels of PAK4/PAK5/PAK6 only when phosphorylated at Ser474.

靶标信息

PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction. PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Activation of GPR40 attenuates neuroinflammation and improves neurological function via PAK4/CREB/KDM6B pathway in an experimental GMH rat model.

Journal of neuroinflammation

Xiao J,Cai T,Fang Y,Liu R,Flores JJ,Wang W,Gao L,Liu Y,Lu Q,Tang L,Zhang JH,Lu H,Tang J

PA5-64495 was used in Western Blot to suggest that GPR40/PAK4/CREB/KDM6B signaling pathway in microglia plays a role in the attenuation of neuroinflammation after GMH.

Sun Jul 18 00:00:00 EDT 2021

生物信息学

蛋白别名: p21 (CDKN1A)-activated kinase 4; p21 (CDKN1A)-activated kinase 6; p21 (CDKN1A)-activated kinase 7; p21 (RAC1) activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 4; p21 protein (Cdc42/Rac)-activated kinase 6; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A)-activated kinase 4; p21(CDKN1A)-activated kinase 6; p21(CDKN1A)-activated kinase 7; p21-activated kinase 4; p21-activated kinase 5; p21-activated kinase 6; p21-activated kinase 7; p21CDKN1A-activated kinase 7; PAK-4; PAK-5; PAK-6; PAK-7; protein kinase PAK5; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; Serine/threonine-protein kinase PAK 4; Serine/threonine-protein kinase PAK 5; Serine/threonine-protein kinase PAK 6; serine/threonine-protein kinase PAK 7; serine/threonine-protein kinase PAK7

基因别名: 2900083L08Rik; 4732456M09; 5730488L07Rik; 6430627N20; AW555722; KIAA1142; KIAA1264; mKIAA1142; PAK-5; PAK-7; PAK4; PAK5; PAK6; PAK7

UniProt ID: (Human) O96013, (Human) Q9P286, (Human) Q9NQU5, (Mouse) Q8BTW9, (Mouse) Q3ULB5, (Rat) D4A280, (Mouse) Q8C015

Entrez Gene ID: (Human) 10298, (Human) 57144, (Human) 56924, (Mouse) 70584, (Rat) 292756, (Mouse) 214230, (Rat) 296078, (Rat) 311450, (Mouse) 241656

分子生物学功能: non-receptor serine/threonine protein kinase