Invitrogen

Phospho-PKC delta (Thr505) Polyclonal Antibody

查看其他29个PKC delta抗体

Product Image Gallery

Phospho-PKC delta (Thr505) Antibody in Western Blot (WB) — Western blot was performed using Anti-Phospho-PKC delta (Thr505) Polyclonal Antibody(Product # PA5-17903) and a 77kDa band corresponding to Phospho-PKC delta (Thr505) was observed across cell lines tested. Whole Cell Extract-WCL (30 µg lysate) of NIH/3T3 (Lane 1), NIH/3T3 treated with PMA(400nM for 30mins) and Calyculin A(10nM for 30min) (Lane 2), U-937 (Lane 3), U-937 treated with PMA (0.2uM for 30min) (Lane 4), later lanes were treated with phosphatase enzyme were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

产品信息

PA5-17903

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:1,000	-

产品规格
种属反应	Human, Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic phosphopeptide corresponding to residues around pThr505 of mouse PKCd
偶联物	Unconjugated
形式	Liquid
纯化类型	Antigen affinity chromatography
保存液	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物	no preservative
保存条件	-20°C
RRID	AB_10980340

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with the phosphorylated forms of PKC isoforms -alpha, -beta, -gamma, -delta or -epsilon.

靶标信息

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: 1-May; kinase nPKC-delta; KPCD; nPKC-delta; Protein kinase C delta type; Protein kinase C delta type catalytic subunit; Protein kinase C delta type regulatory subunit; protein kinase C delta variant IX; protein kinase C delta VIII; protein kinase C, delta; protein kinase C, delta IV; protein kinase C, delta V; protein kinase C[d]; SDK1; Sphingosine-dependent protein kinase-1; Tyrosine-protein kinase PRKCD

基因别名: AI385711; ALPS3; CVID9; D14Ertd420e; MAY1; nPKC-delta; PKC[d]; PKCD; PKCdelta; PRKCD

UniProt ID: (Human) Q05655, (Mouse) P28867

Entrez Gene ID: (Human) 5580, (Mouse) 18753

分子生物学功能: non-receptor serine/threonine protein kinase

stimulatory C-type lectin receptor signaling pathway protein phosphorylation apoptotic process cellular component disassembly involved in execution phase of apoptosis cell cycle signal transduction activation of phospholipase C activity intrinsic apoptotic signaling pathway in response to oxidative stress regulation of receptor activity immunoglobulin mediated immune response histone phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation peptidyl-tyrosine phosphorylation termination of signal transduction platelet activation negative regulation of actin filament polymerization positive regulation of endodeoxyribonuclease activity negative regulation of protein binding activation of protein kinase activity interleukin-10 production interleukin-12 production positive regulation of superoxide anion generation regulation of actin cytoskeleton organization negative regulation of glial cell apoptotic process positive regulation of protein dephosphorylation Fc-gamma receptor signaling pathway involved in phagocytosis B cell proliferation neutrophil activation positive regulation of protein import into nucleus defense response to bacterium negative regulation of MAP kinase activity regulation of mRNA stability negative regulation of insulin receptor signaling pathway negative regulation of inflammatory response negative regulation of peptidyl-tyrosine phosphorylation protein stabilization negative regulation of filopodium assembly cell chemotaxis interferon-gamma-mediated signaling pathway cellular response to hydrogen peroxide cellular response to hydroperoxide negative regulation of platelet aggregation cellular senescence positive regulation of phospholipid scramblase activity cellular response to angiotensin positive regulation of ceramide biosynthetic process positive regulation of glucosylceramide catabolic process positive regulation of sphingomyelin catabolic process positive regulation of response to DNA damage stimulus positive regulation of apoptotic signaling pathway response to oxidative stress aspartate transport phosphorylation collagen metabolic process cellular response to oxidative stress intracellular signal transduction regulation of phosphorylation positive regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of MAPK cascade positive regulation of glucose import protein autophosphorylation

参与通路

protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-independent protein kinase C activity non-membrane spanning protein tyrosine kinase activity protein binding ATP binding enzyme activator activity enzyme binding kinase binding protein kinase binding insulin receptor substrate binding metal ion binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups TIR domain binding