Invitrogen

Phospho-PLCG1 (Tyr783) Recombinant Rabbit Monoclonal Antibody (C-p1p2-39H16L83)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 篇参考文献

查看其他31个PLCG1抗体

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Phospho-PLCG1 (Tyr783) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Phospho-PLCG1 (Tyr783) was performed using 70% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-PLCG1 (Tyr783) Antibody (C-p1p2-39H16L83), Recombinant Rabbit Monoclonal (Product # 700044) at 2 µg/mL in 0.1% BSA and incubated overnight at 4°C and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2,000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel f represents cells treated with antagonist, Afatinib (1 µM for 6 hrs) followed by EGF (200 ng/mL for 10 minutes), showing reduced Phospho-PLCG2 (Tyr1217) staining. Panel e shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Phospho-PLCG1 (Tyr783) Antibody in Western Blot (WB) — Western blot analysis of Phospho-PLC gamma pTyr783 in untreated NIH-3T3 cell lysate or treated with 50 ng/mL PDGF for 30 min (lanes 2-4) using a Phospho-PLC gamma pTyr783 recombinant rabbit monoclonal antibody (Product # 700044).

Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of Phospho-PLCG1 (Tyr783) using Phospho-PLCG1 (Tyr783) Antibody, Recombinant Rabbit Monoclonal (Product # 700044) shows induction of PLCG1 phosphorylation at Tyr783 residue in A-431 cell line upon EGF treatment. Pre-treatment with antagonist, Afatinib resulted in inhibition of EGFR phosphorylation upon EGF treatment. {TM}

产品信息

700044

应用	建议稀释比	已发表文章
免疫印迹 (WB)	2 µg/mL	查看2篇已发表文章 2篇已发表文章
免疫细胞化学 (ICC/IF)	2 µg/mL	-
免疫沉淀 (IP)	-	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human, Mouse
已发表种属	Mouse, Rat
宿主/亚型	Rabbit / IgG
Expression System	EXPI293
分类	Recombinant Monoclonal
类型	Antibody
克隆号	C-p1p2-39H16L83
抗原	A peptide corresponding to amino acids 777-789 of P19174. 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Protein A
保存液	PBS
内含物	0.09% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2532275

产品详细信息

This antibody is predicted to react with bovine, mouse, rat, Xenopus and zebrafish based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Glucocorticoid receptors recruit the CaMKIIα-BDNF-CREB pathways to mediate memory consolidation.

Nature neuroscience

Chen DY,Bambah-Mukku D,Pollonini G,Alberini CM

700044 was used in Western Blotting to examine the molecular mechanisms underlying the role of glucocorticoids in memory consolidation.

Sat Dec 01 00:00:00 EST 2012

Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival.

Proceedings of the National Academy of Sciences of the United States of America

Zikherman J,Doan K,Parameswaran R,Raschke W,Weiss A

Tue Jan 03 00:00:00 EST 2012

Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival.

Proceedings of the National Academy of Sciences of the United States of America

Zikherman J,Doan K,Parameswaran R,Raschke W,Weiss A

Tue Jan 03 00:00:00 EST 2012

生物信息学

蛋白别名: 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; cell differentiation and embryonic development; EC 3.1.4.11; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; OTTHUMP00000178982; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; phospholipase C, gamma 1 (formerly subtype 148); phospholipase C-1; phospholipase C-148; Phospholipase C-gamma-1; Phospholipase C-II; Phospholipase C2; PLC gamma1; PLC-148; PLC-gamma-1; PLC-II; PLC2I; triphosphoinositide phosphodiesterase

基因别名: AI894140; Cded; NCKAP3; Plc-1; Plc-gamma1; PLC-II; PLC1; PLC148; Plcg-1; PLCG1; PLCgamma1

UniProt ID: (Human) P19174, (Mouse) Q62077

Entrez Gene ID: (Human) 5335, (Mouse) 18803

分子生物学功能: phospholipase