Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫组化(石蜡) (IHC (P)) |
1:50-1:100 | - |
免疫细胞化学 (ICC/IF) |
1:100-1:200 | - |
产品规格 | |
---|---|
种属反应 |
Human, Mouse, Rat |
宿主/亚型 |
Rabbit / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
抗原 |
Peptide sequence around phosphorylation site of serine 380(R-Y-S(p)-D-T) derived from Human PTEN. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
1 mg/mL |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.4, with 50% glycerol |
内含物 |
0.02% sodium azide |
保存条件 |
-20°C |
RRID |
AB_2554313 |
The antibody detects endogenous level of PTEN only when phosphorylated at serine 380.
A suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.
PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The PTEN gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. PTEN plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Also, PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form of PTEN induces less tumor suppressive ability.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; phosphatase and tensin homolog deleted on chromosome ten; phosphatase and tensin-like protein; phosphatidylinositol 3; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; protein tyrosine phosphatase and tensin-like protein; pten tumor suppressor
基因别名: 10q23del; 2310035O07Rik; A130070J02Rik; AI463227; B430203M17Rik; BZS; CWS1; DEC; GLM2; MHAM; Mmac; MMAC1; PTEN; PTEN1; rCG_47874; TEP1
UniProt ID: (Human) P60484, (Rat) O54857, (Mouse) O08586
Entrez Gene ID: (Human) 5728, (Rat) 50557, (Mouse) 19211
分子生物学功能:
protein phosphatase