Invitrogen

Phospho-PTPRA (Tyr789) Polyclonal Antibody

Phospho-PTPRA (Tyr789) Antibody in Western Blot (WB)

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Phospho-PTPRA (Tyr789) Antibody (PA5-97409) in WB

Western blot analysis of Phospho-PTPRA (Tyr789) in extracts of C2C12 and PC-12 cells. Samples were incubated with Phospho-PTPRA (Tyr789) Polyclonal antibody (Product # PA5-97409) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. C2C12 cell lysate were treated by CIP (20 µL CIP for each 400 µL cell lysate) at 37°C for 1 hour. PC-12 cell lysa... View More

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产品信息

PA5-97409

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic phosphorylated peptide around Y789 of human PTPRA (NP_5430301)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.01 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2809211

产品详细信息

Positive Samples: C2C12, PC-12; Cellular Location: Membrane, Single-pass type I membrane protein

Immunogen sequence: SDYAN

靶标信息

Phosphorylation of receptors by protein kinases is a process that can be reversed by a group of enzymes called protein phosphatases. Coordinated control of kinases and phosphatases provides the cell with the capacity to rapidly switch between phosphorylated and dephosphorylated protein states in dynamic response to environmental stimuli. Activation of critical enzymes by kinase phosphorylation alone is not enough to provide adequate regulation - it is the combination with phosphatase dephosphorylation that effectively creates on/off switches to control cellular events. Errors in control, either through kinases or their counterpart phosphatases, can lead to unchecked cell growth attributable to human cancers and developmental disorders. Potential mechanisms to control dephosphorylation include changes in the expression of protein phosphatases, their subcellular localization, phosphorylation of phosphatase catalytic and regulatory subunits and regulation by endogenous phosphatase inhibitors. Most protein phosphatases are not stringently specific for their substrates. Consequently, changes in phosphatase activity may have a broad impact on dephosphorylation and turnover of phosphoproteins that are substrates for different kinases. This may be an important point of control to connect cellular circuitry of interrelated signaling pathways, and to synchronize physiological responses.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Hptp alpha; LCA-related phosphatase; Leukocyte common antigen-related peptide (protein tyrosine phosphate); protein tyrosine phosphatase alpha; protein tyrosine phosphatase, receptor type, A; protein tyrosine phosphatase, receptor type, alpha polypeptide; Protein-tyrosine phosphatase alpha; PTP alpha; PTPase-alpha; PTPLCA-related phosphatase; PTPTY-28; R-PTP-alpha; Receptor-type tyrosine-protein phosphatase alpha; RPTP alpha; tyrosine phosphatase alpha

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基因别名: HEPTP; HLPR; HPTPA; HPTPalpha; LRP; PTPA; Ptpalpha; PTPRA; PTPRL2; R-PTP-alpha; RPTPA; Rptpalpha; Rptra; Rptralpha

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UniProt ID: (Human) P18433, (Mouse) P18052

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Entrez Gene ID: (Human) 5786, (Mouse) 19262, (Rat) 25167

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功能