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Phospho-PTPRA (Tyr789) Antibody (PA5-97409) in WB
Western blot analysis of Phospho-PTPRA (Tyr789) in extracts of C2C12 and PC-12 cells. Samples were incubated with Phospho-PTPRA (Tyr789) Polyclonal antibody (Product # PA5-97409) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. C2C12 cell lysate were treated by CIP (20 µL CIP for each 400 µL cell lysate) at 37°C for 1 hour. PC-12 cell lysa... View More
Product Details
PA5-97409
产品规格
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
A phospho specific peptide corresponding to residues surrounding Y789 of human PTPRA.
Conjugate
Unconjugated
形式
Liquid
浓度
0.01 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2809211
Product Specific Information
Positive Samples: C2C12, PC-12; Cellular Location: Membrane, Single-pass type I membrane protein
Target Information
Phosphorylation of receptors by protein kinases is a process that can be reversed by a group of enzymes called protein phosphatases. Coordinated control of kinases and phosphatases provides the cell with the capacity to rapidly switch between phosphorylated and dephosphorylated protein states in dynamic response to environmental stimuli. Activation of critical enzymes by kinase phosphorylation alone is not enough to provide adequate regulation - it is the combination with phosphatase dephosphorylation that effectively creates on/off switches to control cellular events. Errors in control, either through kinases or their counterpart phosphatases, can lead to unchecked cell growth attributable to human cancers and developmental disorders. Potential mechanisms to control dephosphorylation include changes in the expression of protein phosphatases, their subcellular localization, phosphorylation of phosphatase catalytic and regulatory subunits and regulation by endogenous phosphatase inhibitors. Most protein phosphatases are not stringently specific for their substrates. Consequently, changes in phosphatase activity may have a broad impact on dephosphorylation and turnover of phosphoproteins that are substrates for different kinases. This may be an important point of control to connect cellular circuitry of interrelated signaling pathways, and to synchronize physiological responses.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: Hptp alpha; LCA-related phosphatase; Leukocyte common antigen-related peptide (protein tyrosine phosphate); protein tyrosine phosphatase alpha; protein tyrosine phosphatase, receptor type, A; protein tyrosine phosphatase, receptor type, alpha polypeptide; Protein-tyrosine phosphatase alpha; PTP alpha; PTPase-alpha; PTPLCA-related phosphatase; PTPTY-28; R-PTP-alpha; Receptor-type tyrosine-protein phosphatase alpha; RPTP alpha; tyrosine phosphatase alpha
Gene Aliases: HEPTP; HLPR; HPTPA; HPTPalpha; LRP; PTPA; Ptpalpha; PTPRA; PTPRL2; R-PTP-alpha; RPTPA; Rptpalpha; Rptra; Rptralpha
UniProt ID: (Human) P18433, (Mouse) P18052
Entrez Gene ID: (Human) 5786, (Mouse) 19262, (Rat) 25167
Molecular Function:
protein phosphatase
protein modifying enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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