Invitrogen

Phospho-Paxillin (Ser178) Recombinant Rabbit Monoclonal Antibody (21H7L6)

Phospho-Paxillin (Ser178) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Paxillin (Ser178) Antibody (702386) in ICC/IF

For immunofluorescence analysis, MDA-MB-231 cells were fixed and permeabilized for detection of endogenous Paxillin pS (178) using Anti-Paxillin pS (178) Recombinant Rabbit Monoclonal Antibody (Product # 702386, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were sta... View More

Phospho-Paxillin (Ser178) Antibody in Western Blot (WB)

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产品信息

702386

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

21H7L6

抗原

Peptide corresponding to Human PXN (aa 173-181)

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偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2633014

产品详细信息

This antibody is predicted to react with Monkey, Cat and Mouse

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。