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Invitrogen
Phospho-RPH3A (Ser234) Polyclonal Antibody
Product Details
PA1-4693
Applications
Tested Dilution
Publications
产品规格
Species Reactivity
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of rat RPH3A conjugated to KLH
Conjugate
Unconjugated
形式
Liquid
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2181147
Product Specific Information
This antibody is predicted to react with mouse based on 100% sequence homology.
In Western blot, this antibody is specific for the ~82 kDa rabphilin protein phosphorylated at Ser234. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Target Information
The carboxy-terminal of rabphilin-3A consists of two C2 domains, A and B, and the amino-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of other carboxyterminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2. Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. Rabphilin-3A binds Rab3a/GTP/Mg+ within amino-terminal residues 45 and 170. Rabphilin-3A binds calcium ions and phosphatidylinositol 4,5-bisphophate containing lipid vesicles within its C2 domains. Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis. Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other. Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
Bioinformatics
Protein Aliases: Exophilin 1; Exophilin-1; Exophilin1; rabphilin 3A homolog; Rabphilin-3A
Gene Aliases: Rph3a
UniProt ID: (Rat) P47709
Entrez Gene ID: (Rat) 171039
transporter activity
calcium ion binding
protein binding
phospholipid binding
calcium-dependent phospholipid binding
phosphatidylinositol-4,5-bisphosphate binding
zinc ion binding
selenium binding
Rab GTPase binding
protein complex binding
phosphate ion binding
metal ion binding
inositol 1,4,5 trisphosphate binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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