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Invitrogen
Phospho-RSK Pan (Ser221, Ser227, Ser218, Ser232) Recombinant Superclonal Antibody
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
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Phospho-RSK Pan (Ser221, Ser227, Ser218, Ser232) Antibody (711336) in WB
Western blot analysis was performed on Whole cell extracts (30 µg lysate) of A-431 (Lane 1) and A-431 treated with PMA (100 ng/mL for 30 mins) (Lane 2). The blots were probed with Anti-RSK1 (pS221)/RSK2 (pS227)/RSK3 (pS218)/RSK4 (pS232) Recombinant Rabbit Polyclonal Antibody (Product # 711336, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibo... View More
产品信息
711336
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
抗原
A synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/218/223
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633010
产品详细信息
This antibody is predicted to react with Monkey, Rat and Mouse
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
The p90 ribosomal S6 kinases (RSKs) comprise a family of serine/threonine kinases that lie at the terminus of the ERK pathway. In humans, the RSK family consists of four isoforms (RSK1 to-4). RSK family members are unusual among serine/threonine kinases in that they contain two distinct kinase domains, both of which are catalytically functional. Theses kinase domains are activated in a sequential manner by a series of phosphorylations. RSK regulates gene expression via association and phosphorylation of transcriptional regulators including c-Fos, estrogen receptor, NFkappaB/IkappaB alpha, cAMP-response element-binding protein (CREB). ERK activates the C-terminal kinase of RSK, leading to activation of the N-terminal kinase. Members of the RSK family are present in the cytoplasm as well as the nucleus. Addition of growth factor to the cells results in the activation of both cytosolic and nuclear RSK and the translocation of the cytosolic RSK into the nucleus upon activation. The activation and nuclear translocation of RSK result in phosphorylation and activation of transcription factors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 90 kDa ribosomal protein S6 kinase 1; 90 kDa ribosomal protein S6 kinase 2; 90 kDa ribosomal protein S6 kinase 3; 90 kDa ribosomal protein S6 kinase 4; 90 kDa ribosomal protein S6 kinase 6; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); Insulin-stimulated protein kinase 1; ISPK-1; MAP kinase-activated protein kinase 1a; MAP kinase-activated protein kinase 1b; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1a; MAPK-activated protein kinase 1b; MAPK-activated protein kinase 1c; MAPKAP kinase 1a; MAPKAP kinase 1b; MAPKAP kinase 1C; MAPKAPK-1a; MAPKAPK-1b; MAPKAPK1D; mitogen- and stress-activated protein kinase 2; mitogen-activated protein kinase-activated protein kinase 1C; Nuclear mitogen- and stress-activated protein kinase 2; p90 rsk; p90-RSK 1; p90-RSK 2; p90-RSK 3; p90-RSK 6; p90RSK1; p90RSK6; p90S6K; pp90RSK2; pp90RSK3; pp90RSK4; Ribosomal protein kinase B; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase alpha-2; Ribosomal protein S6 kinase alpha-3; Ribosomal protein S6 kinase alpha-4; Ribosomal protein S6 kinase alpha-6; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 2; ribosomal protein S6 kinase, 90kDa, polypeptide 3; ribosomal protein S6 kinase, 90kDa, polypeptide 4; ribosomal protein S6 kinase, 90kDa, polypeptide 6; Ribosomal S6 kinase 1; Ribosomal S6 kinase 2; Ribosomal S6 kinase 3; Ribosomal S6 kinase 4; RP11-393H10.3; RSK-1; RSK-2; RSK-3; RSK-4; RSKB; S6K-alpha 1; S6K-alpha 6; S6K-alpha-1; S6K-alpha-2; S6K-alpha-3; S6K-alpha-4; S6K-alpha-6
基因别名: CLS; HU-1; HU-2; HU-3; ISPK-1; ISPK1; MAPKAPK1A; MAPKAPK1B; MAPKAPK1C; MRX19; MSK2; p90-RSK2; p90-RSK3; p90Rsk; p90RSK2; pp90RSK2; pp90RSK3; PP90RSK4; RPS6KA1; RPS6KA2; RPS6KA3; RPS6KA4; RPS6KA6; RSK; RSK-B; RSK1; RSK2; RSK3; RSK4; S6K-alpha; S6K-alpha-4; S6K-alpha2; S6K-alpha3
UniProt ID: (Human) Q15418, (Human) Q15349, (Human) P51812, (Human) O75676, (Human) Q9UK32
Entrez Gene ID: (Human) 6195, (Human) 6196, (Human) 6197, (Human) 8986, (Human) 27330
magnesium ion binding
protein serine/threonine kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
kinase activity
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
ribosomal protein S6 kinase activity
protein kinase activity
protein kinase binding
histone kinase activity (H3-S10 specific)
mitogen-activated protein kinase p38 binding
protein modifying enzyme
non-receptor serine/threonine protein kinase
protein phosphorylation
apoptotic process
cell cycle
signal transduction
positive regulation of cell growth
intracellular signal transduction
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of translation in response to stress
regulation of DNA-templated transcription in response to stress
positive regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
hepatocyte proliferation
positive regulation of hepatic stellate cell activation
oocyte maturation
brain renin-angiotensin system
heart development
negative regulation of cell proliferation
positive regulation of gene expression
cardiac muscle cell apoptotic process
peptidyl-serine phosphorylation
positive regulation of apoptotic process
negative regulation of cell cycle
negative regulation of meiotic nuclear division
heart contraction
regulation of protein processing
cellular response to carbohydrate stimulus
skeletal system development
toll-like receptor signaling pathway
central nervous system development
response to lipopolysaccharide
negative regulation of cytokine production
regulation of transcription, DNA-templated
inflammatory response
histone phosphorylation
positive regulation of CREB transcription factor activity
positive regulation of histone phosphorylation
positive regulation of histone acetylation
histone H3-S10 phosphorylation
histone H3-S28 phosphorylation
positive regulation of NF-kappaB transcription factor activity
interleukin-1-mediated signaling pathway
DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
negative regulation of embryonic development
negative regulation of ERK1 and ERK2 cascade
negative regulation of mesoderm development
