Invitrogen

Phospho-RSK1 (Ser380) Polyclonal Antibody

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Phospho-RSK1 (Ser380) Antibody in Western Blot (WB) — Western Blot was performed using Anti-Phospho-RSK1 (Ser380) Polyclonal Antibody (Product # PA5-17900) and a 80 kDa band corresponding to Phosphorylated Ribosomal protein S6 kinase alpha-1 was observed. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with PMA (Over-night serum starvation followed by treatment with 25 ng/mL PMA for 20 mins) (Lane 2) and HeLa treated with EGF (Over-night serum starvation followed by treatment with 100 nM EGF for 10 mins) (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Up-regulation in the expression of phosphorylated RSK1 was observed in the lanes treated with PMA and EGF as expected (https://doi.org/10.1038/onc.2012.472, doi: 10.1091/mbc.E11-07-0658).

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

1:1,000

免疫沉淀 (IP)

1:200

产品规格

种属反应

Dog, Hamster, Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phosphopeptide corresponding to residues surrounding pSer380 of human p90RSK

偶联物

Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

RRID

AB_11009263

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPK1A; p90-RSK 1; p90RSK1; p90S6K; pp90RSK1; ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase polypeptide 1; ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RSK-1; S6 protein kinase (Rsk-1); S6K-alpha 1; S6K-alpha-1

基因别名: HU-1; Mapkapk-1a; MAPKAPK1A; p90-Rsk1; p90Rsk; p90Rsk1; p90S6K; RPS6KA1; RSK; Rsk-1; RSK1; S6K-alpha-1

UniProt ID: (Human) Q15418, (Rat) Q63531, (Mouse) P18653

Entrez Gene ID: (Human) 6195, (Dog) 487353, (Chinese hamster) 100770849, (Rat) 81771, (Mouse) 20111

分子生物学功能: protein modifying enzyme