Invitrogen

Phospho-RSK1 (Thr573) Polyclonal Antibody

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Phospho-RSK1 (Thr573) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Phospho-p90RSK pThr573 in HeLa cells, TPA-treated, using a Phospho-p90RSK pThr573 polyclonal antibody (Product # PA5-17841) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Phospho-RSK1 (Thr573) Antibody in Western Blot (WB) — Western blot analysis of Phospho-p90RSK (pThr573) was performed by loading 20 µg of HEK293T whole cell lysates, from cells either left untreated (Lane 1) or induced with 100 nM PMA (Lane 2) for 15 minutes, per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBST for 1 hour at room temperature. The membrane was probed with a Phospho-p90RSK pThr573 polyclonal antibody (Product # PA5-17841) at a dilution of 1:250 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.

产品信息

PA5-17841

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:1,000	-
免疫细胞化学 (ICC/IF)	1:400	-

产品规格
种属反应	Chicken, Hamster, Human, Mouse, Rat, Xenopus, Zebrafish
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic phosphopeptide corresponding to residues surrounding pThr573 of human p90RSK
偶联物	Unconjugated
形式	Liquid
纯化类型	Antigen affinity chromatography
保存液	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物	no preservative
保存条件	-20°C
RRID	AB_10989467

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); MAP kinase-activated protein kinase 1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1; MAPK-activated protein kinase 1a; MAPKAP kinase 1; MAPKAP kinase 1a; MAPKAPK-1; MAPKAPK-1a; MAPKAPK1A; P90-RSK; p90-RSK 1; p90RSK1; p90S6K; pp90RSK1; Ribosomal protein S6 kinase 2 alpha; ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase II alpha; ribosomal protein S6 kinase II beta; ribosomal protein S6 kinase polypeptide 1; ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1 L homeolog; Ribosomal S6 kinase 1; RSK-1; S6 kinase II beta; S6 protein kinase (Rsk-1); S6K-alpha 1; S6K-alpha-1; S6KII-alpha; S6KII-beta

基因别名: HU-1; Mapkapk-1a; MAPKAPK1A; p90-Rsk1; p90Rsk; p90Rsk1; p90S6K; RPS6KA; RPS6KA1; RPS6KA1L; RSK; Rsk-1; RSK1; S6K-alpha-1; wu:fc56h07; XELAEV_18012109mg; zgc:152654

UniProt ID: (Chicken) P18652, (Human) Q15418, (Rat) Q63531, (Mouse) P18653

Entrez Gene ID: (Chicken) 386579, (Human) 6195, (Chinese hamster) 100770849, (Rat) 81771, (Mouse) 20111, (Zebrafish) 777728, (Xenopus) 445821

分子生物学功能: non-receptor serine/threonine protein kinase protein modifying enzyme