Invitrogen

Phospho-SMAD1/SMAD5/SMAD9 (Ser463, Ser465, Ser467) Recombinant Rabbit Monoclonal Antibody (RM487)

Phospho-SMAD1/SMAD5/SMAD9 (Ser463, Ser465, Ser467) Antibody in Western Blot (WB)

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Phospho-SMAD1/SMAD5/SMAD9 (Ser463, Ser465, Ser467) Antibody (MA562161) in WB

Western Blot analysis of mouse liver tissue lysate using Phospho-SMAD1/SMAD5/SMAD9 (Ser463, Ser465, Ser467) recombinant monoclonal antibody (Product # MA562161) at 1:1,000 dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5 and residue Ser465/467 of Smad9.

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产品信息

MA562161

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

RM487

抗原

A phospho-peptide corresponding to human Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

纯化类型

Protein A

保存液

PBS with 1% BSA, 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

This antibody reacts to Smad1 and Smad5 when phosphorylated at Ser463/465 and Smad9 (Smad8) when phosphorylated at Ser465/467. There is no cross-reactivity to Smad1, Smad5, or Smad9 that are not phosphorylated. This antibody reacts to human, mouse or rat Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467).

靶标信息

The protein encoded by SMAD1/SMAD5/SMAD8/SMAD9 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: BSP-1; JV4-1; JV5-1; MAD homolog 1; MAD homolog 5; MAD homolog 9; MAD, mothers against decapentaplegic homolog 1; MAD, mothers against decapentaplegic homolog 5; Mad-related protein 1; Madh6; Mothers against decapentaplegic homolog 1; Mothers against decapentaplegic homolog 5; Mothers against decapentaplegic homolog 9; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against decapentaplegic, drosophila, homolog of, 9; mothers against DPP homolog 1; SMA- and MAD-related protein 5; SMAD; SMAD 1; SMAD 5; SMAD 9; SMAD family member 1; SMAD family member 5; SMAD family member 9; SMAD, mothers against DPP homolog 1; SMAD, mothers against DPP homolog 5; SMAD, mothers against DPP homolog 9; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1

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基因别名: BSP-1; BSP1; DWFC; JV4-1; JV41; JV5-1; MADH1; MADH5; MADH6; MADH9; MADR1; PPH2; SMAD1; SMAD5; SMAD8; SMAD8/9; SMAD8A; SMAD8B; SMAD9

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UniProt ID: (Human) Q15797, (Human) Q99717, (Human) O15198

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Entrez Gene ID: (Human) 4086, (Human) 4090, (Human) 4093

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