Invitrogen

Phospho-SMAD2 (Ser465, Ser467) Recombinant Superclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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产品信息

710612

应用
建议稀释比
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免疫印迹 (WB)

1-2 µg/mL
-

免疫细胞化学 (ICC/IF)

3-5 µg/mL
-
产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

抗原

Synthetic peptide corresponding to human SMAD2, aa 461-467, phosphorylated at Serine 465/467
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532745

产品详细信息

This antibody is predicted to react with mouse, bovine, zebrafish and drosophila based on sequence homology.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

靶标信息

SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: hMAD-2; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MGC22139; MGC34440; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2Deltaexon3

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基因别名: hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2; SMAD2

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UniProt ID: (Human) Q15796

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Entrez Gene ID: (Human) 4087

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功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding transcription factor binding phosphatase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity ubiquitin protein ligase binding activating transcription factor binding type I transforming growth factor beta receptor binding enhancer binding protein homodimerization activity SMAD binding metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding R-SMAD binding DNA-binding transcription factor gene-specific transcriptional regulator
参与通路
negative regulation of transcription from RNA polymerase II promoter ureteric bud development in utero embryonic development endoderm formation mesoderm formation transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly zygotic specification of dorsal/ventral axis gastrulation negative regulation of cell proliferation response to glucose post-embryonic development anterior/posterior pattern specification positive regulation of epithelial to mesenchymal transition regulation of transforming growth factor beta receptor signaling pathway signal transduction involved in regulation of gene expression insulin secretion lung development negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of BMP signaling pathway pancreas development primary miRNA processing activin receptor signaling pathway somatic stem cell population maintenance organ growth intracellular signal transduction nodal signaling pathway cell fate commitment negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of binding palate development pericardium development SMAD protein signal transduction response to cholesterol positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry
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