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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • STAT3 Antibodies

          Invitrogen

          Phospho-STAT3 (Ser727) Polyclonal Antibody

          高级验证
          5个已发表图片
          5 篇参考文献
          查看其他82个STAT3抗体

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          说明书
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          问题与答案
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          说明书
          实验方案
          问题与答案
          技术支持

          Cite Phospho-STAT3 (Ser727) Polyclonal Antibody

          • 抗体检测数据 (4)
          • 已发表图片 (5)
          • 高级验证 (3)
          Phospho-STAT3 (Ser727) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-STAT3 (Ser727) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 12

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          Phospho-STAT3 (Ser727) Antibody (PA5-17876) in ICC/IF

          Immunofluorescence analysis of Phospho-STAT3 (Ser727) was performed using 90% confluent log phase NIH/3T3 cells treated with IL6 (100 ng/mL, 30 mins). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-STAT3 (Ser727) Polyclonal Antibody (Product # PA5-1... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          Phospho-STAT3 (Ser727) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-STAT3 (Ser727) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-STAT3 (Ser727) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-STAT3 (Ser727) Antibody in ChIP Assay (ChIP)
          Phospho-STAT3 (Ser727) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          Phospho-STAT3 (Ser727) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-STAT3 (Ser727) Antibody in Western Blot (WB)
          Phospho-STAT3 (Ser727) Antibody in Western Blot (WB)
          Phospho-STAT3 (Ser727) Antibody in Western Blot (WB)
          Phospho-STAT3 (Ser727) Antibody
          Phospho-STAT3 (Ser727) Antibody
          Phospho-STAT3 (Ser727) Antibody

          产品信息

          PA5-17876

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000
          查看2篇已发表文章 2篇已发表文章

          免疫细胞化学 (ICC/IF)

          1:100
          查看1篇已发表文章 1篇已发表文章

          流式细胞分析 (Flow)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫沉淀 (IP)

          1:50
          -

          染色质免疫沉淀 实验 (ChIP)

          4 µl/10^6 cells
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Human, Mouse, Rat

          已发表种属

          Human

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          Synthetic phosphopeptide corresponding to residues surrounding pSer727 of mouse Stat3
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          53 µg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          内含物

          no preservative

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10980044

          产品详细信息

          It is not recommended to aliquot this antibody.

          靶标信息

          STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: acute phase response factor; Acute-phase response factor; DNA-binding protein APRF; FLJ20882; MGC16063; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); STAT

          查看更多 收起

          基因别名: 1110034C02Rik; ADMIO; ADMIO1; APRF; AW109958; HIES; STAT3

          查看更多 收起

          UniProt ID: (Human) P40763, (Rat) P52631, (Mouse) P42227

          查看更多 收起

          Entrez Gene ID: (Human) 6774, (Rat) 25125, (Mouse) 20848

          查看更多 收起

          功能
          RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding signal transducer activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding protein kinase binding protein phosphatase binding chromatin DNA binding CCR5 chemokine receptor binding glucocorticoid receptor binding identical protein binding transcription regulatory region DNA binding protein dimerization activity RNA polymerase II transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding DNA-binding transcription factor
          参与通路
          negative regulation of transcription from RNA polymerase II promoter temperature homeostasis eye photoreceptor cell differentiation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein import into nucleus movement of cell or subcellular component acute-phase response signal transduction JAK-STAT cascade nervous system development aging cell proliferation negative regulation of cell proliferation positive regulation of gene expression negative regulation of hydrogen peroxide biosynthetic process viral process phosphorylation cytokine-mediated signaling pathway sexual reproduction intracellular receptor signaling pathway response to estradiol cellular response to hormone stimulus leptin-mediated signaling pathway somatic stem cell population maintenance miRNA mediated inhibition of translation regulation of multicellular organism growth response to drug positive regulation of tyrosine phosphorylation of Stat3 protein glucose homeostasis eating behavior negative regulation of apoptotic process cellular response to leptin stimulus response to leptin response to ethanol positive regulation of Notch signaling pathway negative regulation of glycolytic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of mitochondrial membrane permeability astrocyte differentiation regulation of cell cycle radial glial cell differentiation regulation of feeding behavior growth hormone receptor signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway interleukin-6-mediated signaling pathway cellular response to organic cyclic compound energy homeostasis negative regulation of neuron death positive regulation of growth factor dependent skeletal muscle satellite cell proliferation positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of metalloendopeptidase activity positive regulation of gene silencing by miRNA positive regulation of ATP biosynthetic process negative regulation of neuron migration response to organic substance response to organic cyclic compound stem cell population maintenance response to cytokine response to peptide hormone negative regulation of cell death transcription, DNA-templated
          It has to be done as per old AB suggested Products section.
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