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Invitrogen
Phospho-STAT6 (Tyr641) Monoclonal Antibody (CHI2S4N), PE-Cyanine7, eBioscience™
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Phospho-STAT6 (Tyr641) Antibody (25-9013-42) in Flow
U937 cells were unstimulated (orange histogram) or stimulated for 15 minutes with Human IL-4 Recombinant Protein (Product # 200-04-50UG) (purple histogram). Cells were then stained intracellularly, using the Intracellular Fixation/Methanol protocol, with 0.25 ug Anti-Human/Mouse phospho-STAT6 (Y641) PE-Cyanine7. Viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product ... View More
产品信息
25-9013-42
产品规格
种属反应
Human,
Mouse
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
CHI2S4N
偶联物
激发/发射光谱
569/780 nm
查看光谱
形式
liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Description
This CHI2S4N monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 6 (STAT6) when phosphorylated on tyrosine 641. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to IL-4, STAT6 is phosphorylated on tyrosine 641 by JAK1 and JAK3. STAT6 signaling downstream of the IL-4 receptor promotes T cell growth and B cell production of IgE. Specificity of this CHI2S4N clone was determined by ELISA and flow cytometry.
Applications Tested: This CHI2S4N antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity:
This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
靶标信息
STAT6 protein is a transcription factor activated by cytokines, particularly interleukin-4 and IL13. (Stat6-/-) were found to be deficient in IL-4-mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 immune response. STAT6 is crucial for IL-4-mediated biological responses, and naturally in two isoforms: STAT6a and STAT6c.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 12S1644; IL-4 Stat; IL4 STAT; Signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; Signal transducer and transcription activator 6; STAT; STAT, interleukin4-induced; STAT-6; transcription factor IL-4 STAT
基因别名: D12S1644; IL-4-STAT; STAT6; STAT6B; STAT6C
UniProt ID: (Human) P42226, (Mouse) P52633
Entrez Gene ID: (Human) 6778, (Mouse) 20852
negative regulation of transcription from RNA polymerase II promoter
T-helper 1 cell lineage commitment
negative regulation of type 2 immune response
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
signal transduction
positive regulation of type I interferon production
mammary gland epithelial cell proliferation
interleukin-4-mediated signaling pathway
regulation of cell proliferation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
mammary gland morphogenesis
cellular response to hydrogen peroxide
cellular response to reactive nitrogen species
regulation of transcription, DNA-templated
cytokine-mediated signaling pathway
response to cytokine
