Invitrogen

Phospho-eIF4E (Ser209) Recombinant Rabbit Monoclonal Antibody (RM452)

Phospho-eIF4E (Ser209) Antibody in Western Blot (WB)

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Phospho-eIF4E (Ser209) Antibody (MA562157) in WB

Western Blot analysis of the lysates of MCF-7 cells nontreated (-) or treated (+) with fetal bovine serum using Phospho-eIF4E (Ser209) recombinant monoclonal antibody (Product # MA562157) at 1:1,000 dilution.

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产品信息

MA562157

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

RM452

抗原

A phospho-peptide corresponding to human Phospho-eIF4E (Ser209).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

纯化类型

Protein A

保存液

PBS with 1% BSA, 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

This antibody reacts to human eIF4E only when phosphorylated at Ser209. There is no cross-reactivity to eIF4E that is not phosphorylated. This antibody may also react to mouse or rat Phospho-eIF4E (Ser209) as predicted by immunogen homology.

靶标信息

eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: eIF-4E; eIF-4F 25 kDa subunit; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705

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基因别名: AUTS19; CBP; eIF-4E; EIF4E; EIF4E1; EIF4EL1; EIF4F

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UniProt ID: (Human) P06730

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Entrez Gene ID: (Human) 1977

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功能