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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • mTOR Antibodies

          Invitrogen

          Phospho-mTOR (Ser2448) Polyclonal Antibody

          高级验证
          4个已发表图片
          17 篇参考文献
          查看其他47个mTOR抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite Phospho-mTOR (Ser2448) Polyclonal Antibody

          • 抗体检测数据 (3)
          • 已发表图片 (4)
          • 高级验证 (1)
          Phospho-mTOR (Ser2448) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-mTOR (Ser2448) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 8

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          Phospho-mTOR (Ser2448) Antibody (44-1125G) in ICC/IF

          Immunofluorescence analysis of Phospho-mTOR/FRAP pSer2448 was done on 70% confluent log phase A549 cells treated with serum starvation followed by 200 ng of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with of Phospho-mTOR/FRAP pSer2448 Rab... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          Phospho-mTOR (Ser2448) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
          Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
          Phospho-mTOR (Ser2448) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
          Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
          Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
          Phospho-mTOR (Ser2448) Antibody

          产品信息

          44-1125G

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000
          查看13篇已发表文章 13篇已发表文章

          免疫组化 (IHC)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫组织化学(多聚甲醛固定) (IHC (PFA))

          -
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          1:250
          查看1篇已发表文章 1篇已发表文章

          流式细胞分析 (Flow)

          -
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Human, Mouse

          已发表种属

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human mTOR that contains serine 2448. The sequence is conserved in human, mouse and rat.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          纯化类型

          Antigen affinity chromatography

          保存液

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          内含物

          0.05% sodium azide

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533580

          靶标信息

          FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; mTORC1; Rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR

          查看更多 收起

          基因别名: 2610315D21Rik; AI327068; flat; FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS

          查看更多 收起

          UniProt ID: (Human) P42345, (Mouse) Q9JLN9

          查看更多 收起

          Entrez Gene ID: (Human) 2475, (Mouse) 56717

          查看更多 收起

          功能
          RNA polymerase III type 1 promoter DNA binding RNA polymerase III type 2 promoter DNA binding RNA polymerase III type 3 promoter DNA binding TFIIIC-class transcription factor binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity protein kinase binding protein domain specific binding ribosome binding phosphoprotein binding nucleotide binding drug binding transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor protein dimerization activity non-receptor serine/threonine protein kinase protein modifying enzyme
          参与通路
          negative regulation of protein phosphorylation positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation heart morphogenesis heart valve morphogenesis regulation of glycogen biosynthetic process energy reserve metabolic process 'de novo' pyrimidine nucleobase biosynthetic process DNA repair protein phosphorylation response to stress cell cycle arrest signal transduction germ cell development brain development cell aging response to nutrient long-term memory visual learning post-embryonic development negative regulation of autophagy positive regulation of lamellipodium assembly positive regulation of gene expression positive regulation of myotube differentiation positive regulation of neuron maturation negative regulation of muscle atrophy cell growth macroautophagy negative regulation of macroautophagy phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation spinal cord development protein catabolic process positive regulation of actin filament polymerization T cell costimulation negative regulation of protein ubiquitination ruffle organization regulation of myelination cellular response to nutrient levels TOR signaling regulation of fatty acid beta-oxidation regulation of response to food response to insulin regulation of actin cytoskeleton organization social behavior multicellular organism growth growth wound healing response to cocaine regulation of GTPase activity response to amino acid response to morphine regulation of carbohydrate utilization positive regulation of nitric oxide biosynthetic process regulation of osteoclast differentiation positive regulation of translation negative regulation of cell size regulation of protein kinase activity positive regulation of transcription from RNA polymerase III promoter protein autophosphorylation positive regulation of lipid biosynthetic process phosphatidylinositol-mediated signaling mRNA stabilization positive regulation of smooth muscle cell proliferation positive regulation of oligodendrocyte differentiation positive regulation of peptidyl-tyrosine phosphorylation voluntary musculoskeletal movement positive regulation of stress fiber assembly negative regulation of NFAT protein import into nucleus positive regulation of protein kinase B signaling cardiac muscle cell development cardiac muscle contraction maternal process involved in female pregnancy positive regulation of glial cell proliferation positive regulation of dendritic spine development positive regulation of cell growth involved in cardiac muscle cell development cellular response to hypoxia regulation of brown fat cell differentiation regulation of membrane permeability regulation of cellular response to heat positive regulation of neuron death positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter positive regulation of eating behavior positive regulation of cholangiocyte proliferation positive regulation of sensory perception of pain negative regulation of cholangiocyte apoptotic process positive regulation of granulosa cell proliferation positive regulation of skeletal muscle hypertrophy negative regulation of iodide transmembrane transport double-strand break repair via homologous recombination regulation of carbohydrate metabolic process cell projection organization
          It has to be done as per old AB suggested Products section.
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