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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • p53 Antibodies

          Invitrogen

          Phospho-p53 (Ser20) Polyclonal Antibody

          Advanced Verification

          This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

          1 Reference
          View all (102) p53 antibodies
          Datasheet
          Protocols
          Tech Support

          Cite Phospho-p53 (Ser20) Polyclonal Antibody

          Datasheet
          Protocols
          Tech Support
          • Antibody Testing Data (1)
          • Advanced Verification (1)
          Phospho-p53 (Ser20) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Phospho-p53 (Ser20) Antibody (PA5-17894) in ICC/IF

          Immunofluorescence analysis of Phospho-p53 (Ser20) was performed using 70% confluent log phase HCT116 cells treated with etoposide. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-p53(Ser20) Polyclonal Antibody (Product # PA5-17894) at 1:100 dilution... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Product Details

          PA5-17894

          Applications
          Tested Dilution
          Publications

          免疫印迹 (WB)

          1:1,000
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          1:100
          -
          产品规格

          Species Reactivity

          Human, Non-human primate

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          Immunogen

          Synthetic phosphopeptide corresponding to residues surrounding pSer20 of human p53
          View immunogen

          Conjugate

          Unconjugated

          形式

          Liquid

          纯化类型

          Antigen affinity chromatography

          保存液

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          内含物

          no preservative

          保存条件

          -20°C

          RRID

          AB_10981312

          Product Specific Information

          It is not recommended to aliquot this antibody.

          This antibody is not cross-reactive with p53 phosphorylated at other sites.

          Target Information

          The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          Bioinformatics

          Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; FLJ92943; HGNC11998; mutant tumor protein 53; OTTMUSP00000006194; p53 tumor suppressor; Phosphoprotein p53; transformation-related protein 53; tumor protein 53; Tumor suppressor p53; tumor supressor p53; Tumour Protein p53

          View more View less

          Gene Aliases: BCC7; LFS1; P53; TP53; TRP53

          View more View less

          UniProt ID: (Human) P04637

          View more View less

          Entrez Gene ID: (Human) 7157

          View more View less

          Molecular Function: P53-like transcription factor

          Function(s)
          negative regulation of transcription from RNA polymerase II promoter DNA strand renaturation base-excision repair nucleotide-excision repair regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein complex assembly cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator ER overload response cell cycle arrest Ras protein signal transduction multicellular organism development cell aging protein localization cell proliferation negative regulation of cell proliferation determination of adult lifespan response to X-ray response to gamma radiation positive regulation of gene expression viral process protein sumoylation cell differentiation negative regulation of cell growth DNA damage response, signal transduction by p53 class mediator positive regulation of histone deacetylation chromatin assembly mitotic G1 DNA damage checkpoint positive regulation of protein oligomerization cellular response to UV cellular response to drug cellular response to glucose starvation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process entrainment of circadian clock by photoperiod proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of neuron apoptotic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter response to antibiotic positive regulation of protein export from nucleus regulation of mitochondrial membrane permeability negative regulation of fibroblast proliferation circadian behavior positive regulation of peptidyl-tyrosine phosphorylation negative regulation of helicase activity protein tetramerization negative regulation of telomerase activity positive regulation of thymocyte apoptotic process positive regulation of cell cycle arrest cellular response to hypoxia cellular response to ionizing radiation intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of release of cytochrome c from mitochondria replicative senescence oxidative stress-induced premature senescence intrinsic apoptotic signaling pathway oligodendrocyte apoptotic process positive regulation of execution phase of apoptosis positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway regulation of signal transduction by p53 class mediator regulation of cell cycle G2/M phase transition positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress positive regulation of reactive oxygen species metabolic process positive regulation of intrinsic apoptotic signaling pathway
          Process(es)
          RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protease binding p53 binding DNA binding chromatin binding damaged DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding copper ion binding protein binding ATP binding transcription factor binding zinc ion binding enzyme binding protein kinase binding protein phosphatase binding receptor tyrosine kinase binding ubiquitin protein ligase binding histone acetyltransferase binding identical protein binding sequence-specific DNA binding protein self-association transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding chaperone binding protein phosphatase 2A binding
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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