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Progen
Plakophilin 2 Monoclonal Antibody (Pkp2-518)
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产品信息
651167
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
Pkp2-518
抗原
Synthetic Carboxyterminal Peptide (Aa 527 - 872) Of Human Plakophilin 2
偶联物
形式
Liquid
浓度
Conc. Not Determined
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Specificity: plakophilin 2 in both locations: in the desmosomal plaque and in the karyoplasm. Plakophilin 2 is a member of the arm-repeat family of proteins which can occur in at least 2 splice forms (2a and 2b). Plakophilin 2 assembles with other proteins to form the desmosomal plaque in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, in Merkel cells, and desmosome-possessing nonepithelial cells such as myocardium, Purkinje fibers, and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, plakophilin 2 is highly enriched in the nuclei (karyoplasm). Polypeptide reacting: Plakophilin 2, MW ~100 kD (SDS-PAGE); MW calculated from aa sequence: 92,750 (pI 9.33) and 97,410 (pI 9.38)Positive staining was obtained with several carcinomas derived from simple-type epithelia (e.g. hepatomas, colon carcinomas) and some adenocarcinomas derived from stratified epithelia (oesophagus carcinoma).Recommendation for immunohistochemistry on frozen sections: For better accessibility of the desmosome-associated antigen frozen sections might be pre-incubated for 5 min in PBS plus 0.2% Triton X-100 prior to antibody application. Longer incubation could result in a loss of Pkp2-nuclear staining. To prevent wash-out of soluble forms of the antigen, fixation with 2% formaldehyde is recommended.
靶标信息
Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 1 mediates increases in desmosomal protein content, desmosome assembly and regulation of cell migration. Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart. Plakophilin 4 is a component of desmosomal adhesion plaques that regulates junctional plaque organization and cadherin function.
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生物信息学
蛋白别名: Plakophilin-2
基因别名: 1200008D14Rik; 1200012P04Rik; AA516617; ARVD9; PKP2; Pkp2l
UniProt ID: (Human) Q99959, (Mouse) Q9CQ73, (Rat) F1M7L9
Entrez Gene ID: (Human) 5318, (Mouse) 67451, (Rat) 287925
molecular_function
protein kinase C binding
protein binding
sodium channel regulator activity
intermediate filament binding
protein complex scaffold
ion channel binding
alpha-catenin binding
cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication
cadherin binding involved in cell-cell adhesion
intermediate filament binding protein
desmosome assembly
heart development
negative regulation of cell proliferation
positive regulation of sodium ion transport
gap junction assembly
single organismal cell-cell adhesion
negative regulation of cell migration
adherens junction maintenance
intermediate filament bundle assembly
maintenance of organ identity
ventricular cardiac muscle tissue morphogenesis
lipid homeostasis
cardiac muscle cell action potential
cardiac muscle cell action potential involved in contraction
ventricular cardiac muscle cell action potential
cell-cell signaling involved in cardiac conduction
cell communication by electrical coupling involved in cardiac conduction
bundle of His cell-Purkinje myocyte adhesion involved in cell communication
regulation of heart rate by cardiac conduction
establishment of protein localization to plasma membrane
regulation of ventricular cardiac muscle cell action potential
regulation of bicellular tight junction assembly
bundle of His cell to Purkinje myocyte communication
