MUbio

Plakophilin-3 Monoclonal Antibody (12B11F8)

Name: Plakophilin-3 Monoclonal Antibody (12B11F8)
Brand: MUbio
SKU: MUB1502P

查看其他8个PKP3抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

产品信息

MUB1502P

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:200

免疫细胞化学 (ICC/IF)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Dog, Human, Mouse, Pig

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

12B11F8

抗原

Plakophilin-3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

12B11F8 reacts with an epitope located between residues 779-793 in Human plakophilin-3.

The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

靶标信息

Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalization, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localization in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of ~87 kDa.

仅用于科研。不用于诊断过程。未经明确授权不得转售。