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Invitrogen
Prolactin Receptor Monoclonal Antibody (U5)
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Prolactin Receptor Antibody (MA1-610) in IHC
Mammary gland tissue from lactating light horse mare (equine) was incubated in MA1-610 anti-prolactin receptor monoclonal mouse antibody at 1:100 for 1 hour at 23°C and for 24 hours at 4°C. Second antibody was biotinylated goat anti-mouse IgG at 23°C for 1 hour. Development using avidin-biotin complex 23°C for 1 hour plus 3, 3-diaminobenzadine for 15 min and counterstained with haematoxylin. Notes: Antigen ... View More
产品信息
MA1-610
应用
建议稀释比
已发表文章
产品规格
种属反应
Horse,
Human,
Mouse,
Pig,
Rabbit,
Rat
已发表种属
Baboon,
Bovine,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
U5
抗原
Purified rat liver PRL receptor.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-610 detects prolactin (PRL) receptor from rat, human, rabbit, horse, and pig tissues.
MA1-610 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and FACS procedures. By Western blot, this antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. Immunohistochemical staining of PRL receptor in rat Islets of Langerhans with MA1-610 results in a staining pattern consistent with cytoplasmic vesicle staining. MA1-610, after biotinylation, has been used to detect human lymphocyte PRL receptor by FACS. MA1-610 does not inhibit the interaction of prolactin with the receptor.
The MA1-610 immunogen is purified PRL receptor from rat liver. This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain. MA1-610 does not inhibit the interaction of prolactin with the receptor.
Reconstitute with 100 µL PBS.
靶标信息
Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cytokine receptor; hPRL receptor; Lactogen receptor; Prl R; PRL-R; Prolactin receptor; prolactin receptor 2 precursor; prolactin receptor related sequence 1; prolactin receptor short isoform; secreted prolactin binding protein
基因别名: AI987712; HPRL; hPRLrI; MFAB; pPRLR-SF; Pr-1; Pr-3; PRLR; Prlr-rs1; RATPRLR
UniProt ID: (Rabbit) P14787, (Pig) Q6JTA8, (Human) P16471, (Rat) P05710, (Mouse) Q08501
Entrez Gene ID: (Rabbit) 100009046, (Pig) 414916, (Human) 5618, (Horse) 100053793, (Rat) 24684, (Mouse) 19116
prolactin signaling pathway
oncostatin-M-mediated signaling pathway
leukemia inhibitory factor signaling pathway
steroid biosynthetic process
cell surface receptor signaling pathway
activation of transmembrane receptor protein tyrosine kinase activity
embryo implantation
lactation
regulation of cell adhesion
regulation of epithelial cell differentiation
T cell activation
activation of JAK2 kinase activity
negative regulation of apoptotic process
JAK-STAT cascade involved in growth hormone signaling pathway
mammary gland epithelial cell differentiation
prostate gland growth
mammary gland alveolus development
JAK-STAT cascade
cytokine-mediated signaling pathway
mammary gland epithelium development
