Invitrogen

Protein Z Polyclonal Antibody

Name: Protein Z Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-120422

查看其他9个Protein Z抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

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Protein Z Antibody (PA5-120422) in WB

Western blot analysis of Protein Z in extracts of various cell lines. Samples were incubated with Protein Z Polyclonal antibody (Product # PA5-120422) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

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Immunogen image

Immunogen Image Protein Z Polyclonal Antibody

产品信息

PA5-120422

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 220-340 of human PROZ (NP_003882.1).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.48 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2913994

产品详细信息

Positive test controls include: HepG2, 293T, Mouse kidney, Rat liver, Rat kidney. The target is usually found in the following locations: Secreted.

Immunogen sequence: SLLHRNITVK TYFNRTSQDP LMIKITHVHV HMRYDADAGE NDLSLLELEW PIQCPGAGLP VCTPEKDFAE HLLIPRTRGL LSGWARNGTD LGNSLTTRPV TLVEGEECGQ VLNVTVTTRT Y

靶标信息

PROZ protein Z, vitamin K-dependent plasma glycoprotein. It is 62 kDa large and 396 amino acids long. It has four domains: a gla-rich region, two EGF-like domains and a trypsin-like domain. It lacks the serine residue that would make it catalytically active as a serine protease. It is a member of the coagulation cascade, the group of blood proteins that leads to the formation of blood clots. It is vitamin K-dependent, and its functionality is therefore impaired in warfarin therapy. It is a glycoprotein. Although it is not enzymatically active, it is structurally related to several serine proteases of the coagulation cascade: factors VII, IX, X and protein C. The carboxyglutamate residues (which require vitamin K) bind protein Z to phospholipid surfaces. The main role of protein Z appears to be the degradation of factor Xa. This is done by protein Z-related protease inhibitor (ZPI), but the reaction is accelerated 1000-fold by the presence of protein Z. Oddly, ZPI also degrades factor XI, but this reaction does not require the presence of protein Z. In some studies, deficiency states have been associated with a propensity to thrombosis. Others, however, link it to bleeding tendency; there is no clear explanation for this, as it acts physiologically as an inhibitor, and deficiency would logically have led to a predisposition for thrombosis.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。