Invitrogen

Prothrombin Polyclonal Antibody

Name: Prothrombin Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-120155

查看其他26个Prothrombin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Prothrombin Antibody in Western Blot (WB)

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Prothrombin Antibody (PA5-120155) in WB

Western blot analysis of Prothrombin in extracts of HepG2 cells. Samples were incubated with Prothrombin Polyclonal antibody (Product # PA5-120155) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

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Immunogen image

Immunogen Image Prothrombin Polyclonal Antibody

产品信息

PA5-120155

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 364-622 of human F2 (NP_000497.1).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.519 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2913727

产品详细信息

Positive test controls include: HepG2. The target is usually found in the following locations: Secreted, extracellular space.

Immunogen sequence: IVEGSDAEIG MSPWQVMLFR KSPQELLCGA SLISDRWVLT AAHCLLYPPW DKNFTENDLL VRIGKHSRTR YERNIEKISM LEKIYIHPRY NWRENLDRDI ALMKLKKPVA FSDYIHPVCL PDRETAASLL QAGYKGRVTG WGNLKETWTA NVGKGQPSVL QVVNLPIVER PVCKDSTRIR ITDNMFCAGY KPDEGKRGDA CEGDSGGPFV MKSPFNNRWY QMGIVSWGEG CDRDGKYGFY THVFRLKKWI QKVIDQFGE

靶标信息

Prothrombin or Coagulation factor II, is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。